Structural basis of proteolytic activation of L-phenylalanine oxidase from Pseudomonas sp. P-501

The Journal of Biological Chemistry
Koh IdaHaruo Suzuki

Abstract

The mature form of l-phenylalanine oxidase (PAOpt) from Pseudomonas sp. P-501 was generated and activated by the proteolytic cleavage of a noncatalytic proenzyme (proPAO). The crystal structures of proPAO, PAOpt, and the PAOpt-o-amino benzoate (AB) complex were determined at 1.7, 1.25, and 1.35A resolutions, respectively. The structure of proPAO suggests that the prosequence peptide of proPAO occupies the funnel (pathway) of the substrate amino acid from the outside of the protein to the interior flavin ring, whereas the funnel is closed with the hydrophobic residues at its vestibule in both PAOpt and the PAOpt-AB complex. All three structures have an oxygen channel that is open to the surface of the protein from the flavin ring. These results suggest that structural changes were induced by proteolysis; that is, the proteolysis of proPAO removes the prosequence and closes the funnel to keep the active site hydrophobic but keeps the oxygen channel open. The possibility that an interaction of the hydrophobic side chain of substrate with the residues of the vestibule region may open the funnel as a putative amino acid channel is discussed.

References

Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Feb 1, 1994·Archives of Biochemistry and Biophysics·E B MukouyamaH Koyama
Sep 5, 1993·Journal of Molecular Biology·L Holm, C Sander
May 6, 1998·Protein Science : a Publication of the Protein Society·A R Khan, M N James
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·T C Terwilliger, J Berendzen
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
May 20, 1999·Nature Structural Biology·A PerrakisV S Lamzin
Aug 16, 2000·Acta Crystallographica. Section D, Biological Crystallography·T C Terwilliger
Sep 22, 2001·Acta Crystallographica. Section D, Biological Crystallography·A A Vagin, M N Isupov
Dec 12, 2002·Chemical Reviews·Philip N Bryan
Aug 20, 2003·Acta Crystallographica. Section D, Biological Crystallography·Cristofer Enroth
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Aug 4, 2004·Journal of Molecular Biology·B Martin HallbergChristina Divne
Mar 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·G J Kleywegt, T A Jones
Aug 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Enrico MalitoAndrea Mattevi
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·E Krissinel, K Henrick
Apr 22, 2005·Journal of Molecular Biology·Qingqiu HuangQuan Hao
Apr 8, 2006·Trends in Biochemical Sciences·Andrea Mattevi
Dec 22, 2006·Biotechnology Journal·Jirí DamborskýMichal Otyepka
Jan 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Andrea AlfieriAndrea Mattevi
Dec 25, 2007·Acta Crystallographica. Section A, Foundations of Crystallography·George M Sheldrick

❮ Previous
Next ❯

Citations

Oct 1, 2013·Applied Microbiology and Biotechnology·Loredano PollegioniGianluca Molla
Dec 24, 2013·Archives of Biochemistry and Biophysics·Mieke M E HuijbersWillem J H van Berkel
Feb 27, 2009·Antioxidants & Redox Signaling·Toshiya SendaTetsuo Ishida
Mar 24, 2009·Journal of Biochemistry·Nami KurosawaHaruo Suzuki
Dec 20, 2013·Applied Microbiology and Biotechnology·Gazi Sakir HossainJian Chen
Apr 8, 2011·The FEBS Journal·Tse Siang KangR Manjunatha Kini
Jan 19, 2018·Proceedings of the National Academy of Sciences of the United States of America·Ying HuangSteven G Van Lanen
Jun 13, 2020·The Journal of Applied Laboratory Medicine·Junhua ZhangFei Xiao
Jul 29, 2016·The Journal of Biological Chemistry·Janis J FüllerJürgen Moser
Feb 16, 2021·Biotechnology Advances·Caroline E PaulWillem J H van Berkel
Nov 19, 2018·Current Opinion in Chemical Biology·Yasuhisa Asano, Kazuyuki Yasukawa
Jul 3, 2021·The Clinical Respiratory Journal·Pengbo YangXiaomao Xu

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.