Structural basis of RNA binding discrimination between bacteriophages Qbeta and MS2

Structure
Wilf T HornP G Stockley

Abstract

Sequence-specific interactions between RNA stem-loops and coat protein (CP) subunits play vital roles in the life cycles of the RNA bacteriophages, e.g., by allowing translational repression of their replicase cistrons and tagging their own RNA genomes for encapsidation. The CPs of bacteriophages Qbeta and MS2 each discriminate in favor of their cognate translational operators, even in the presence of closely related operators from other phages in vivo. Discrete mutations within the MS2 CP have been shown to relax this discrimination in vitro. We have determined the structures of eight complexes between such mutants and both MS2 and Qbeta stem-loops with X-ray crystallography. In conjunction with previously determined in vivo repression data, the structures enable us to propose the molecular basis for the discrimination mechanism.

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Citations

Nov 6, 2007·BMC Biochemistry·Yulia Benitex, Anne M Baranger
Dec 11, 2007·Nature Structural & Molecular Biology·Jeffrey A ChaoRobert H Singer
Nov 27, 2015·Journal of Molecular Biology·Óttar RolfssonPeter G Stockley
Jan 27, 2012·Current Opinion in Structural Biology·Jeffrey A Speir, John E Johnson
Nov 17, 2009·Journal of Molecular Biology·E C DykemanR Twarock
Nov 17, 2009·Journal of Molecular Biology·Gabriella BasnakPeter G Stockley
Sep 13, 2008·Journal of Molecular Biology·Magnus PerssonLars Liljas
May 3, 2008·Journal of Molecular Biology·Alexandra Shulman-PelegHaim J Wolfson
Dec 1, 2007·Journal of Molecular Biology·Katerina ToropovaNeil A Ranson
Apr 17, 2007·Journal of Molecular Biology·Peter G StockleyAlison E Ashcroft
Feb 21, 2013·RNA Biology·Alexander BorodavkaPeter G Stockley
Jun 29, 2016·Small·Zhuo ChenJeremiah J Gassensmith
Mar 23, 2013·Acta Crystallographica. Section D, Biological Crystallography·Magnus PerssonLars Liljas
Jul 27, 2012·Biomacromolecules·Jason D FiedlerM G Finn
Apr 6, 2019·Annual Review of Biophysics·Reidun Twarock, Peter G Stockley
Oct 29, 2017·Proceedings of the National Academy of Sciences of the United States of America·Zhicheng CuiJunjie Zhang
Apr 3, 2020·Biochemistry·Jiannan Fu, Kenneth J Woycechowsky

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