Structural Behavior of the Endothelial Glycocalyx Is Associated With Pathophysiologic Status in Septic Mice: An Integrated Approach to Analyzing the Behavior and Function of the Glycocalyx Using Both Electron and Fluorescence Intravital Microscopy

Anesthesia and Analgesia
Hanae KataokaTakehiko Iijima

Abstract

The endothelial surface layer (ESL) regulates vascular permeability to maintain fluid homeostasis. The glycocalyx (GCX), which has a complex and fragile ultrastructure, is an important component of the ESL. Abnormalities of the GCX have been hypothesized to trigger pathological hyperpermeability. Here, we report an integrated in vivo analysis of the morphological and functional properties of the GCX in a vital organ. We examined the behavior of the ESL and GCX, using both electron microscopy (EM) and intravital microscopy (IVM). We also compared morphological changes in the ESL of mouse skin in a glycosidase-treated and control group. Combined approaches were also used to examine both morphology and function in a lipopolysaccharide-induced septic model and the pathophysiological features of leukocyte-endothelial interactions and in vivo vascular permeability. Using IVM, we identified an illuminated part of the ESL as the GCX and confirmed our observation using morphological and biochemical means. In septic mice, we found that the GCX was thinner than in nonseptic controls in both an EM image analysis (0.98 ± 2.08 nm vs 70.68 ± 36.36 nm, P< .001) and an IVM image analysis (0.36 ± 0.15 μm vs 1.07 ± 0.39 μm, P< .001). Under septic...Continue Reading

References

Nov 1, 1991·Microvascular Research·A VillringerK M Einhäupl
Apr 4, 1998·Acta Anaesthesiologica Scandinavica·M RehmU Finsterer
Sep 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·A W Mulivor, H H Lipowsky
Mar 15, 2003·Circulation Research·Bernard M van den BergJos A E Spaan
Sep 30, 2004·International Immunopharmacology·Moisés A Franco-MolinaCristina Rodríguez-Padilla
Apr 5, 2005·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Fitz-Roy E Curry
Jun 21, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Dirk BrueggerBernhard Friedrich Becker
Jul 21, 2006·Best Practice & Research. Clinical Anaesthesiology·Birgitte Brandstrup
Mar 25, 2009·Current Opinion in Anaesthesiology·Daniel ChappellMatthias Jacob
Jun 30, 2009·The Journal of Surgical Research·Jochen SteppanMarkus A Weigand
Aug 6, 2009·Best Practice & Research. Clinical Anaesthesiology·Matthias JacobMarkus Rehm
Sep 18, 2009·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Lanlan Zhou, Wafik S El-Deiry
Mar 5, 2010·Cardiovascular Research·J Rodney Levick, C Charles Michel
Jan 29, 2011·Journal of Vascular Research·Sietze ReitsmaMarc A M J van Zandvoort
Apr 9, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Eno E EbongJohn M Tarbell
Oct 20, 2011·Annals of Biomedical Engineering·F E Curry, R H Adamson
Mar 23, 2012·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Roger WagnerKirk Czymmek
Jul 17, 2012·Journal of the American Society of Nephrology : JASN·Andrew H J SalmonJanos Peti-Peterdi
Oct 6, 2012·Critical Care : the Official Journal of the Critical Care Forum·Alessio AlfieriZoe L Brookes
Nov 17, 2012·Microvascular Research·Ivo Torres FilhoMichael A Dubick
Jun 5, 2013·Scientific Reports·Gyohei EgawaKenji Kabashima
Aug 31, 2013·Microvascular Research·Abele DonatiMervyn Singer
Jan 30, 2014·Perioperative Medicine·Takehiko IijimaChrister H Svensen
Oct 15, 2014·Anesthesiology·Marcos L MirandaEliete Bouskela
Jan 21, 2015·The Annals of Thoracic Surgery·Dirk BrueggerDaniel Chappell
Apr 19, 2015·Critical Care : the Official Journal of the Critical Care Forum·Cosimo ChelazziChiara Adembri
Jan 16, 2016·Microscopy Research and Technique·Hanae KataokaTakehiko Iijima
Sep 13, 2016·Journal of Intensive Care·Akira UshiyamaTakehiko Iijima

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Citations

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Dec 26, 2018·Journal of Thrombosis and Haemostasis : JTH·T Iba, J H Levy
Mar 5, 2019·Chinese Medical Journal·Rui-Na CaoRui Xia
Feb 27, 2020·American Journal of Respiratory and Critical Care Medicine·Jérémie JoffreHafid Ait-Oufella
Sep 30, 2017·Critical Reviews in Clinical Laboratory Sciences·Vladimir CernyZdenek Zadak
Mar 7, 2020·Pediatric Critical Care Medicine : a Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies·Jaime Fernández-SarmientoJoseph A Carcillo
Apr 9, 2019·Chinese Medical Journal·Rui-Na CaoRui Xia
Feb 26, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Xiao-Lin YiWen-Fu Tang
Apr 4, 2021·International Journal of Molecular Sciences·Valentina MasolaGiovanni Gambaro
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Nov 20, 2021·Frontiers in Cardiovascular Medicine·Shingo MitsudaAkira Ushiyama

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