Structural Biology in the Multi-Omics Era.

Journal of Chemical Information and Modeling
Caitlyn L McCaffertyDavid W Taylor

Abstract

Rapid developments in cryogenic electron microscopy have opened new avenues to probe the structures of protein assemblies in their near native states. Recent studies have begun applying single -particle analysis to heterogeneous mixtures, revealing the potential of structural-omics approaches that combine the power of mass spectrometry and electron microscopy. Here we highlight advances and challenges in sample preparation, data processing, and molecular modeling for handling increasingly complex mixtures. Such advances will help structural-omics methods extend to cellular-level models of structural biology.

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Citations

Mar 22, 2021·The Journal of Biological Chemistry·Fei LiRobert M Stroud
May 4, 2021·Frontiers in Molecular Biosciences·Fotis L KyrilisPanagiotis L Kastritis
Jun 2, 2021·Essays in Biochemistry·Ping YiBert W O'Malley
Aug 19, 2021·Chemical Reviews·Rivkah Rogawski, Michal Sharon
May 27, 2020·Journal of Chemical Information and Modeling·Giulia PalermoRommie E Amaro

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Methods Mentioned

BETA
X-ray
NMR
electron microscopy
two-hybrid

Software Mentioned

cryoID
Segger
GEM
MDFF
in
Map
Fit
PRO
UCSF Chimera

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