Structural, Biosynthetic, and Serological Cross-Reactive Elucidation of Capsular Polysaccharides from Streptococcus pneumoniae Serogroup 16

Journal of Bacteriology
Chengxin LiJens Ø Duus

Abstract

Capsular polysaccharides (CPS) are crucial virulence factors of Streptococcus pneumoniae The previously unknown CPS structures of the pneumococcal serogroup 16 (serotypes 16F and 16A) were thoroughly elucidated by nuclear magnetic resonance (NMR) spectroscopy and verified by chemical analysis. The following repeat unit structures were determined: 16F, -3)-α-l-Rhap-[4-P-1-Gro]-(1-3)-α-d-Glcp-[(6-P-1)-Gro]-(1-3)-β-l-Rhap-[2-OAc]-(1-4)-β-d-Glcp-(1-; 16A, -3)-β-d-Galf-[2-OAc (70%)]-(1-3)-α-l-Rhap-(1-2)-α-l-Rhap-(1-3)-α-d-Galp-[(6-P-1)-Gro]-(1-3)-β-d-Galp-(1-4)-β-d-Glcp-(1- (OAc, O-acetyl substitution; P-1-Gro, glycerol-1-phosphate substitution) A further analysis of CPS biosynthesis of serotypes 16F and 16A, in conjunction with published cps gene bioinformatics analysis and structures of related serotypes, revealed presumable specific function of glycosyltransferase, acetyltransferase, phosphotransferase, and polymerase. The functions of glycosyltransferases WcxN and WcxT were proposed for the first time, and they were assigned to catalyze linkage of α-l-Rhap-(1-3)-α-d-Glcp and α-l-Rhap-(1-2)-α-l-Rhap, respectively. Furthermore, since serotype 16F was genetically close to serogroup 28, cross-reactions between serogroup 16 and serog...Continue Reading

References

Jul 1, 1988·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J C Richards, M B Perry
Jul 13, 2000·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Z ZhangW Miller
Nov 1, 1953·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·O H LOWRY
Jun 25, 2005·Bioinformatics·Tim J CarverJulian Parkhill
Mar 15, 2006·PLoS Genetics·Stephen D BentleyBrian G Spratt
Sep 4, 2007·Journal of Bacteriology·Angeliki MavroidiBrian G Spratt
Apr 16, 2008·Journal of Magnetic Resonance·Andreas EnthartBurkhard Luy
Jun 16, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Angelique G S C JansenEelko Hak
Feb 13, 2010·The European Respiratory Journal·M LujánJ Rello
Feb 2, 2011·Analytical Chemistry·Magnus Lundborg, Göran Widmalm
Sep 26, 2013·PloS One·Andreia N HorácioUNKNOWN Portuguese Group for the Study of Streptococcal Infections
Dec 20, 2013·Journal of Clinical Microbiology·Juan J CalixMoon H Nahm
Jun 19, 2015·Clinical Microbiology Reviews·K Aaron GenoMoon H Nahm
May 20, 2016·Carbohydrate Research·Katarzyna A DudaOtto Holst
Feb 17, 2017·Journal of Clinical Microbiology·K Aaron GenoMoon H Nahm
Jul 1, 2017·Journal of Clinical Microbiology·K Aaron GenoJinghua Yang

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