Nov 1, 1983

Structural changes during development in bovine fetal epiphyseal cartilage

Collagen and Related Research
J A Buckwalter, L Rosenberg


Sedimentation coefficients of approximately 150 S show that proteoglycan aggregates from bovine fetal epiphyseal cartilage are exceptionally large. To determine the structural basis for the unusually large size of these proteoglycan aggregates, identify changes in proteoglycan structure with changing developmental age, and provide a basis for demonstrating the structural modifications which may occur in growth plate proteoglycan aggregates during endochondral ossification, we examined the molecular architecture and dimensions of fetal epiphyseal proteoglycans by electron microscopy. The eight bovine epiphyseal cartilages studied ranged in fetal age from 168 to 241 days. Proteoglycans were extracted in 4 M guanidinium hydrochloride containing protease inhibitors and isolated by equilibrium density gradient centrifugation under associative and dissociative conditions. Electron micrographs were made from monolayer preparations of proteoglycan-cytochrome c mixtures on nitrocellulose support films. The overall molecular architecture of the proteoglycan aggregates from fetal epiphyseal cartilages was similar to that of aggregates from other cartilages and showed a single, unbranched central hyaluronic acid filament to which many prot...Continue Reading

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Mentioned in this Paper

Centrifugation, Density Gradient
Protease Inhibitors [MoA]
Structure of Calf of Leg
Keratan Sulfate
Endochondral Ossification
Hyaluronic Acid Test
Cattle calf (organism)
Bos taurus

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