Structural changes in bacteriorhodopsin in purple membranes induced by irreversible photobleaching with heterogeneous and homogeneous stability

Photochemistry and Photobiology
Yasunori YokoyamaShigeki Mitaku

Abstract

Kinetic studies of irreversible photobleaching of bacteriorhodopsin (bR) in purple membrane (PM) at neutral pH have previously indicated the existence of two kinds of species which differ in their structural stability. bR was shown to have kinetically slow- and fast-decayed components with the faster one increasing with changes in intra- and intermolecular structures in darkness. However, our recent work reported that photobleaching kinetics above pH 10 were characterized by a single-decay component. In order to elucidate the factors responsible for the heterogeneous or homogeneous stability of photobleaching, we conducted investigations into the structural changes in bR in PM induced by photobleaching at pH 7 and 11 by attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy. ATR-FTIR spectra of bR photobleached at pH 7 and 11 showed that an increase in IR peak intensity at 1656 cm(-1) occurred simultaneously with decreases at 1666 cm(-1), indicating an alpha(II)-to-alpha(I) transition in transmembrane helices during photobleaching. The most significant change in IR spectra occurred at 1626 cm(-1) for samples photobleached at pH 7, and was attributed to structures formed between adjacent molecules. The or...Continue Reading

References

Jul 9, 1976·Biochimica Et Biophysica Acta·R A BogomolniW Stoeckenius
Mar 1, 1979·Biophysical Journal·K J Rothschild, N A Clark
Jan 1, 1977·Annual Review of Biophysics and Bioengineering·R Henderson
Apr 20, 1979·Science·K J Rothschild, N A Clark
Jun 20, 1990·Journal of Molecular Biology·R HendersonK H Downing
Jan 29, 1988·Biochimica Et Biophysica Acta·W K Surewicz, H H Mantsch
Nov 17, 1987·Biochemistry·C G BrouilletteT K Finney
Jul 3, 1995·FEBS Letters·S G TanevaF M Goñi
Jun 1, 1999·FEBS Letters·Z DancsházyA Dér
Aug 24, 1999·Journal of Molecular Biology·H LueckeJ K Lanyi
Jun 1, 2002·Journal of Biochemistry·Yasunori YokoyamaShigeki Mitaku
Oct 9, 2003·The Journal of Biological Chemistry·Karl EdmanRichard Neutze
Jun 7, 2005·Photochemistry and Photobiology·Takanori SasakiShigeki Mitaku
Aug 7, 2007·Biochemical and Biophysical Research Communications·Núria Benseny-CasesJosep Cladera

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.