Structural changes in base-paired region 28 in 16 S rRNA close to the decoding region of the 30 S ribosomal subunit are correlated to changes in tRNA binding

Journal of Molecular Biology
G EricsonP Wollenzien

Abstract

Escherichia coli 30 S ribosomal subunits undergo a reversible change under low monovalent or divalent cation concentration and become inactive in tRNA binding and 50 S subunit association. In the inactive form, 16 S rRNA base-pairs (921-922).(1395-1396) and (923-925).(1391-1393), which are part of region 28, are unstable and an alternate arrangement, (921-923).(1532-1534), is detected by psoralen photochemical crosslinking. Site-directed mutagenesis has been used to investigate whether changes in base-paired region 28 or the alternate secondary structure is responsible for the inactivity of the subunit. 30 S subunits with the substitution C1533A or with deletion of nucleotides 1534 to 1542 can still be inactivated like the wild-type 30 S subunit. On the other hand, 30 S subunits that contain sequence changes in the 920 to 926 region show moderate to severe decreases in tRNA binding even under activating conditions. When 30 S subunits containing these mutations were subjected to chemical probing, they failed to show the normal hyper-reactivity of nucleotide G926 and, instead, reactivity was shifted to G925 or to G928, and G929. Two mutations in the 920 region result in structures in which A1394 is base-paired rather than being u...Continue Reading

Citations

Jan 1, 1997·Nucleic Acids Research·K L Triman, B J Adams
Mar 5, 2004·Nucleic Acids Research·Tatjana ShapkinaPaul Wollenzien
Jan 1, 1997·Annual Review of Biochemistry·R Green, H F Noller
May 4, 2005·Molecular Cell·Manjuli R SharmaRajendra K Agrawal
Feb 28, 2002·The Journal of Biological Chemistry·Connie YarianPaul F Agris
Jul 1, 2008·Journal of Molecular Biology·Takatsugu KimuraHyouta Himeno

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