PMID: 2495819May 1, 1989Paper

Structural changes in fibrin clot associated with the proteolytic activity induced by tissue type plasminogen activator. An NMR study

Biochimica Et Biophysica Acta
R AzouryE Lavie

Abstract

The kinetic behavior of fibrin clot lysis as induced by tissue-type plasminogen activator (t-PA) was studied using proton magnetic resonance (PMR) and a release assay of fibrin labeled with technetium-99m isotope (99mTc). The lysis pattern of the preformed clot was examined as a function of gradual changes in the amounts of added t-PA and deactivated t-PA. The behavior of fibrinolysis was found to depend strongly on the amount of t-PA in the assay, which markedly affects the lysis rate of fibrin. The changes induced by the lysis were reflected in pronounced prolongation of the transverse relaxation time. The PMR and the radioisotope release measurements point to the possibility that at least two steps are involved in the mechanism of lysis. The PMR seems to be associated with structural features of the clot and reflects the liberation of compartmentalized water from the clot, while the 99mTc analysis reflects the further fragmentation of fibrin.

References

Jan 1, 1979·Progress in Biophysics and Molecular Biology·R Mathur-De Vré
Nov 1, 1986·Journal of the American College of Cardiology·B E Sobel
May 15, 1985·European Journal of Biochemistry·B NorrmanM Rånby
Feb 1, 1984·The International Journal of Applied Radiation and Isotopes·E LavieS Mechlis
Jan 1, 1982·Magnetic Resonance Imaging·G D FullertonN C Dornbluth
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·R M LavinskyD W Rose
Oct 1, 1952·Archives of Biochemistry and Biophysics·T ASTRUP, S MULLERTZ
Sep 22, 2012·Blood·Ronald A AlbrightDemetrios T Braddock

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Citations

Jul 9, 1999·Journal of Biomedical Materials Research·C A Bense, K A Woodhouse
Sep 24, 2004·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·S E Noorjahan, T P Sastry

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