Structural changes in the photoactive site of proteorhodopsin during the primary photoreaction

Biochemistry
Vladislav B BergoKenneth J Rothschild

Abstract

Proteorhodopsin (PR), found in marine gamma-proteobacteria, is a newly discovered light-driven proton pump similar to bacteriorhodopsin (BR). Because of the widespread distribution of proteobacteria in the worldwide oceanic waters, this pigment may contribute significantly to the global solar energy input in the biosphere. We examined structural changes that occur during the primary photoreaction (PR --> K) of wild-type pigment and two mutants using low-temperature FTIR difference spectroscopy. Several vibrations detected in the 3500-3700 cm(-1) region are assigned on the basis of H(2)O --> H(2)(18)O exchange to the perturbation of one or more internal water molecules. Substitution of the negatively charged Schiff base counterion, Asp97, with the neutral asparagine caused a downshift of the ethylenic (C=C) and Schiff base (C=N) stretching modes, in agreement with the 27 nm red shift of the visible lambda(max). However, this replacement did not alter the normal all-trans to 13-cis isomerization of the chromophore or the environment of the detected water molecule(s). In contrast, substitution of Asn230, which is in a position to interact with the Schiff base, with Ala induces a 5 nm red shift of the visible lambda(max) and alters...Continue Reading

References

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Citations

Oct 31, 2007·Journal of Biomolecular NMR·Nicole PflegerClemens Glaubitz
Jan 2, 2013·Journal of Biological Physics·Erica C Saint ClairKenneth J Rothschild
Sep 29, 2011·Journal of the American Chemical Society·Matthew J RanaghanRobert R Birge
Sep 17, 2011·Journal of the American Chemical Society·Meaghan E WardVladimir Ladizhansky
Oct 23, 2010·The Journal of Physical Chemistry. B·Matthew J RanaghanRobert R Birge
Nov 30, 2012·The Journal of Physical Chemistry. B·Erica C Saint ClairKenneth J Rothschild
Aug 23, 2008·The Journal of Physical Chemistry. B·Joel M KraljKenneth J Rothschild
Feb 9, 2010·The Journal of Physical Chemistry. B·Andrei K DioumaevJanos K Lanyi
May 12, 2005·Nature Reviews. Microbiology·Edward F DeLong
Jun 9, 2006·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Leonid S Brown, Kwang-Hwan Jung
Nov 19, 2008·The Journal of Biological Chemistry·Vladislav B BergoJohn L Spudich
Mar 10, 2009·Photochemistry and Photobiology·Gabriela SchäferWerner Mäntele
Feb 5, 2009·Photochemistry and Photobiology·Mirka-Kristin VerhoefenJosef Wachtveitl
Dec 3, 2014·Progress in Nuclear Magnetic Resonance Spectroscopy·Shenlin Wang, Vladimir Ladizhansky
Jun 29, 2012·Biochemistry·Julia HerzJosef Wachtveitl
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Apr 11, 2006·Biophysical Journal·Martin O LenzJosef Wachtveitl
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Feb 1, 2008·Biophysical Journal·Alisa RupenyanMarie Louise Groot
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Feb 10, 2006·FEMS Microbiology Ecology·Naomi Ward
Jan 11, 2017·The Journal of Physical Chemistry Letters·C Elias EckertJosef Wachtveitl
Mar 28, 2007·The Journal of Biological Chemistry·Yuki SudoHideki Kandori
Sep 27, 2019·Photochemistry and Photobiology·Gaoxiang MeiKenneth J Rothschild
Jun 14, 2005·The Journal of Biological Chemistry·Vladislav B BergoJohn L Spudich
Apr 6, 2021·Photochemistry and Photobiology·Gaoxiang MeiKenneth J Rothschild
Apr 22, 2010·Biochemistry·Yamit SharaabiMordechai Sheves
Apr 19, 2019·The Journal of Physical Chemistry. B·Yusaku HontaniJohn T M Kennis
Nov 22, 2014·Journal of the American Chemical Society·Jiafei MaoClemens Glaubitz
Mar 4, 2011·Journal of the American Chemical Society·Franziska HempelmannClemens Glaubitz
Sep 21, 2007·The Journal of Physical Chemistry. B·Jason J AmsdenKenneth J Rothschild

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