Structural characterisation of some fatty acids from the brain as biomarkers of BSE risk material

Analytical and Bioanalytical Chemistry
Wolfgang BiedermannAndreas Hensel

Abstract

Identification of bovine and ovine tissue from the central nervous system (CNS: brain and spinal cord) in meat products is possible by using certain CNS fatty acids as biomarkers in GC-MS analysis. Furthermore, the relationship between the isomers of the tetracosenic acid (C24:1) is important for differentiation of the species and age of the CNS in view of the legal definition of specified risk material (SRM). This has so far been referred to as the "cis/trans ratio of the isomers of nervonic acid"; however, structural analysis was not performed. Here we present results from GC-MS structural analysis by retention time and DMDS adduct profiling of the even numbered monoenoic fatty acids from C18:1 to C26:1. Retention times and mass spectra of the FAME standards indicated that the so far designated trans-nervonic acid has a different isomeric structure in the tetracosenic acid from brain-sample extracts. By performing GC-MS analysis of DMDS adducts we have shown that this isomer was actually cis-17-tetracosenic acid in all species so far tested, not trans-15-tetracosenic acid ( trans-nervonic acid). The tetracosenic acid isomer ratio proved to be species-specific in accordance with previous results. Thus, instead of the ratio of ...Continue Reading

Citations

Feb 9, 2005·Analytical and Bioanalytical Chemistry·Ernst LückerAndreas Hensel
Jul 16, 2008·Journal of Agricultural and Food Chemistry·Holger SchönenbrücherJacob W Petrich

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