Structural characterisation of xyloglucan secreted by suspension-cultured cells of Nicotiana plumbaginifolia

Carbohydrate Research
I M SimsAntony Bacic

Abstract

Linkage analysis of a xyloglucan from the extracellular medium of suspension cultures of Nicotiana plumbaginifolia showed mostly 4-Glcp and 4,6-Glcp, terminal Xylp and 2-Xylp, and terminal Araf, along with approximately 10% (w/w) O-acetyl groups, equivalent to approximately 0.28 mol acetyl per mol of glycosyl residue. Methylation with methyl trifluoromethanesulfonate under neutral conditions, followed by re-methylation with CD3I under basic conditions, and conversion into partially methylated alditol acetates showed that O-acetyl groups were primarily attached to C-6 of approximately 44% of the 4-Glcp backbone not substituted with Xylp residues and to C-5 of approximately 15% of the terminal Araf residues. These positions of the O-acetyl groups were confirmed by 1H-NMR. Oligosaccharides generated by digestion of native xyloglucan with endo-(1-->4)-beta-glucanase were separated by a combination of gel-filtration chromatography and anion-exchange HPLC, and analysed by glycosyl linkage analysis and by electrospray ionisation-mass spectrometry (ESI-MS). The major oligosaccharide subunits were Glc4Xyl2 and Glc5Xyl2, of which 50-60% are substituted with one terminal Araf residue attached to O-2 of a Xylp residue, and a further 20-25%...Continue Reading

References

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Citations

May 19, 2006·Biochemical and Biophysical Research Communications·Didier HatschJean-Marc Jeltsch
Aug 7, 2012·Nature Protocols·Filomena A PettolinoAntony Bacic
Aug 1, 2008·Plant Physiology·Radnaa NaranNicholas C Carpita
Dec 1, 2001·Microbiology and Molecular Biology Reviews : MMBR·R P de Vries, J Visser
Jun 27, 2013·Planta·Markus PaulyGuangyan Xiong
Oct 29, 2015·Journal of Bioscience and Bioengineering·Tayebeh AbediNobukazu Tanaka
May 15, 2015·Journal of Agricultural and Food Chemistry·Arland T HotchkissChristina Khoo
May 15, 2012·Biotechnology Advances·Peter Biely
Aug 31, 2013·Carbohydrate Polymers·Nelson R VinuezaHilkka I Kenttämaa
Jan 1, 2014·Plants·Alex SchultinkMarkus Pauly
Aug 18, 2018·The Plant Journal : for Cell and Molecular Biology·Maria StranneYumiko Sakuragi
Dec 15, 2010·The Plant Journal : for Cell and Molecular Biology·Laurence ChevalierAzeddine Driouich
Mar 23, 2007·The Journal of Biological Chemistry·Tracey M GlosterGideon J Davies
Feb 23, 2017·Carbohydrate Polymers·Snehasish BasuJeffrey M Catchmark

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