Structural characterization and 5'-mononucleotide binding of polyalanine beta-sheet complexes

Journal of Molecular Recognition : JMR
E Pérez-PayáS E Blondelle

Abstract

A study was initiated into the formation and stability of highly soluble beta-sheet macrostructures. Such beta-sheet macrostructures are useful model systems for the study of the biological function of the hydrophobic core of proteins and for the de novo design of novel catalytic mimics. In the current study, a 16-mer-alanine-based peptide (Ac-KA14K-NH2) that is highly water soluble and adopts an extremely stable macromolecular beta-sheet structure was synthesized. A tyrosine-containing analog (Ac-KYA13K-NH1) was used to study the tertiary structure of the complex by circular dichroism spectroscopy, while the influence of the charges on the complex formation and binding affinity was evaluated using a zwitterionic analog (Ac-KEA13KE-NH1). Both the secondary and tertiary structures of the beta-sheet complex were stable to denaturants, as demonstrated by far- and near-ultraviolet circular dichroism spectroscopy. Binding studies with mononucleotides have shown that the beta-sheet complex binds to molecules through both hydrophobic and electrostatic interactions. These intrinsic properties were found to be a prerequisite for the observed enhanced cleavage of phosphodiester bonds.

References

Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S MarquseeR L Baldwin
Jun 1, 1986·International Journal of Peptide and Protein Research·R A HoughtenC J Kirby
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S ZhangA Rich
Jun 6, 1995·Biochemical and Biophysical Research Communications·B ForoodS E Blondelle
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·T P QuinnD C Richardson
Sep 1, 1993·Bioconjugate Chemistry·J Haensler, F C Szoka

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Citations

Dec 12, 2001·Current Opinion in Chemical Biology·R FiammengoD N Reinhoudt
Sep 22, 2011·Biochemistry·Joseph P Bernacki, Regina M Murphy
Dec 10, 2015·PloS One·Michael P FriedmannRoland Riek
Oct 5, 1997·Biopolymers·S E BlondelleE Pérez-Payá
Nov 8, 2014·Soft Matter·Maria-Teodora PopescuConstantinos Tsitsilianis
Jul 14, 1998·Immunotechnology : an International Journal of Immunological Engineering·H R HoogenboomR C Roovers

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