Structural Characterization and Absolute Quantification of Microcystin Peptides Using Collision-Induced and Ultraviolet Photo-Dissociation Tandem Mass Spectrometry

Journal of the American Society for Mass Spectrometry
Troy J AttardGavin E Reid

Abstract

Microcystin (MC) peptides produced by cyanobacteria pose a hepatotoxic threat to human health upon ingestion from contaminated drinking water. While rapid MC identification and quantification in contaminated body fluids or tissue samples is important for patient treatment and outcomes, conventional immunoassay-based measurement strategies typically lack the specificity required for unambiguous determination of specific MC variants, whose toxicity can significantly vary depending on their structures. Furthermore, the unambiguous identification and accurate quantitation of MC variants using tandem mass spectrometry (MS/MS)-based methods can be limited due to a current lack of appropriate stable isotope-labeled internal standards. To address these limitations, we have systematically examined here the sequence and charge state dependence to the formation and absolute abundance of both "global" and "variant-specific" product ions from representative MC-LR, MC-YR, MC-RR, and MC-LA peptides, using higher-energy collisional dissociation (HCD)-MS/MS, ion-trap collision-induced dissociation (CID)-MS/MS and CID-MS3, and 193 nm ultraviolet photodissociation (UPVD)-MS/MS. HCD-MS/MS was found to provide the greatest detection sensitivity for...Continue Reading

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Citations

Jan 13, 2021·Critical Reviews in Analytical Chemistry·P U Ashvin Iresh FernandoLee C Moores
Jun 30, 2019·Journal of the American Society for Mass Spectrometry·David J ForemanScott A McLuckey
Dec 19, 2019·Chemical Reviews·Jennifer S BrodbeltInês Santos
Nov 2, 2019·Analytical Chemistry·Luis A MaciasJennifer S Brodbelt
Dec 8, 2021·Analytical Chemistry·Yadiris GarcíaEduardo D Pereira
Jan 13, 2022·Environmental Science & Technology·David Baliu-RodriguezDragan Isailovic

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