Structural characterization and biological activities of a novel polysaccharide from cultured Cordyceps militaris and its sulfated derivative

Journal of Agricultural and Food Chemistry
Yongshuai JingRongmin Yu

Abstract

A novel polysaccharide (CMPA90-1; compound 1) was isolated from the cultured fruiting bodies of Cordyceps militaris. The chemical structure of compound 1 was elucidated by acid hydrolysis, periodate oxidation, Smith degradation, and methylation analysis, along with Fourier transform infrared spectroscopy, high-performance anion-exchange chromatography coupled with pulsed amperometric detection, gas chromatography-mass spectrometry, and one-dimensional [(1)H and (13)C nuclear magnetic resonance (NMR)] and two-dimensional NMR (heteronuclear single-quantum coherence and heteronuclear multiple-bond correlation). Sulfation of compound 1 by the chlorosulfonic acid-pyridine (CSA-Pyr) method led to synthesis of its sulfated analogue (CMPA90-M1; compound 2). The ultrastructures of both compounds 1 and 2 were further characterized by scanning electron microscopy and atomic force microscopy. The results of antioxidant assays showed that compounds 1 and 2 exhibited free-radical-scavenging effects, ferrous-ion-chelating ability, and reducing power. Also, in the cytotoxicity assay, compounds 1 and 2 showed inhibitory activity against A549 cells, with IC50 values of 39.08 and 17.33 μg/mL, respectively.

References

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Apr 19, 2005·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Yvonne V YuanNatalie A Walsh

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Citations

Feb 20, 2016·International Journal of Biological Macromolecules·Yunfei XuJi Zhang
Aug 26, 2015·Analytical and Bioanalytical Chemistry·Hankun HuYi-Ming Liu
Sep 1, 2015·Journal of Agricultural and Food Chemistry·Qingxia YuanXiaoxiong Zeng
Jul 29, 2020·Critical Reviews in Microbiology·Shengli YangHui Zhang
Jun 16, 2019·The Journal of Microbiology·Xiao-Kui MaJian Liang
Feb 3, 2018·PloS One·Hong-Mei ShangHong-Xin Wu
Feb 26, 2021·Frontiers in Pharmacology·Gitishree DasJayanta Kumar Patra
Jan 20, 2018·Journal of Agricultural and Food Chemistry·Liyuan YunMin Zhang

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