Structural characterization and comparative phylogenetic analysis of Escherichia coli HemK, a protein (N5)-glutamine methyltransferase

Journal of Molecular Biology
Zhe YangXiaodong Cheng

Abstract

Protein glutamine methylation at GGQ sites of protein chain release factors plays a pivotal role in the termination of translation. We report here the crystal structure of the Escherichia coli HemK protein (N5)-glutamine methyltransferase (MTase) in a binary complex with the methyl-donor product S-adenosyl-L-homocysteine (AdoHcy). HemK contains two domains: a putative substrate binding domain at the N terminus consisting of a five helix bundle and a seven-stranded catalytic domain at the C terminus that harbors the binding site for AdoHcy. The two domains are linked by a beta-hairpin. Structure-guided sequence analysis of the HemK family revealed 11 invariant residues functioning in methyl-donor binding and catalysis of methyl transfer. The putative substrate-binding domains of HemK from E.coli and Thermotoga maritima are structurally similar, despite the fact that they share very little sequence similarity. When the two proteins are aligned structurally, the helical N-terminal domain is subject to approximately 10 degrees of hinge movement relative to the C-terminal domain. The apparent hinge mobility of the two domains may reflect functional importance during the reaction cycle. Comparative phylogenetic analysis of the hemK g...Continue Reading

References

Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jul 1, 1987·European Journal of Biochemistry·G MichaelsF E Nargang
Feb 14, 1971·Journal of Molecular Biology·B Lee, F M Richards
Aug 12, 1994·Journal of Molecular Biology·T S KrishnaJ Kuriyan
Sep 5, 1993·Journal of Molecular Biology·L Holm, C Sander
May 28, 1996·Proceedings of the National Academy of Sciences of the United States of America·M W SouthworthF B Perler
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·A R Mushegian, E V Koonin
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·R M Esnouf
Dec 3, 1999·Progress in Biophysics and Molecular Biology·K D Cowtan, K Y Zhang
Mar 18, 2000·Genome Research·G PerrièreM Gouy
May 16, 2000·The Journal of Biological Chemistry·V BoothC H Arrowsmith
Jul 13, 2000·Journal of Molecular Biology·O EmanuelssonG von Heijne
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·B J MayV Kapur
Aug 29, 2001·Bioinformatics·J P Huelsenbeck, F Ronquist
Sep 22, 2001·Acta Crystallographica. Section D, Biological Crystallography·J Navaza
Jan 4, 2003·Nature·Urmila B S RawatJoachim Frank
Apr 26, 2003·Acta Crystallographica. Section A, Foundations of Crystallography·Zbyszek OtwinowskiWladek Minor
Jun 27, 2003·Trends in Biochemical Sciences·Heidi L SchubertXiaodong Cheng
Aug 13, 2003·Bioinformatics·Fredrik Ronquist, John P Huelsenbeck
Aug 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Claudia BindaAndrea Mattevi

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Citations

Apr 9, 2009·Molecular & Cellular Proteomics : MCP·Tanya C Petrossian, Steven G Clarke
Oct 30, 2008·Nucleic Acids Research·Augusto Simoes-BarbosaPatricia J Johnson
Aug 16, 2013·Nucleic Acids Research·Avinash S PunekarMaria Selmer
Dec 26, 2015·Proteomics·Maria Lluch-SenarLuis Serrano
Nov 28, 2015·Science·Wolf HoltkampMarina V Rodnina
Jan 24, 2012·Biochimie·Marc GrailleValérie Heurgué-Hamard
Sep 11, 2007·Journal of Molecular Biology·Chu WangDavid Baker
Nov 2, 2005·Protein Science : a Publication of the Protein Society·Warren SunDinesh Christendat
Aug 3, 2007·Proteins·Chu WangDavid Baker
Jul 6, 2007·Molecular Microbiology·Bogdan Polevoda, Fred Sherman
Sep 7, 2007·Proteins·Kevin WieheZhiping Weng
May 9, 2006·Trends in Biochemical Sciences·Albert JeltschFrank Lyko
Dec 21, 2005·Molecular Cell·Marc GrailleRichard H Buckingham
May 5, 2005·Annual Review of Biophysics and Biomolecular Structure·Xiaodong ChengXing Zhang
Jan 23, 2016·The Journal of Biological Chemistry·Denis KusevicAlbert Jeltsch
May 8, 2019·Nature Structural & Molecular Biology·Eric MetzgerRoland Schüle
Feb 11, 2020·Frontiers in Microbiology·Sarah D Riggs-ShuteZhaomin Yang

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