Structural characterization and immunostimulating activity of a levan-type fructan from Curcuma kwangsiensis

International Journal of Biological Macromolecules
Cai-Xia DongYuan-Xia Sun

Abstract

A fructan designated as CKNP with apparent molecular weight of 5.3kD was isolated from the hot water extract of Curcuma kwangsiensis through a combination of ion-exchange chromatography on DEAE 650M and gel filtration on Superdex G-200. CKNP was characterized by chemical derivatization as well as HPLC, GC, and GC-MS technologies. Structural studies revealed that CKNP is composed predominately of fructose (96.8%) and a small amount of glucose (3.2%) with a degree of polymerization (DP) of 30-31. It was deduced to be a levan-type fructan containing a backbone composed of (2→6)-linked β-d-Fruf residues and single β-d-Fruf residues as side chains branched at the O-1 position along the backbone. Preliminary in vitro bioactive tests on RAW 264.7 murine macrophage cells revealed that the levan-type fructan from C. kwangsiensis shows significant immunostimulating activity based on its ability to stimulate macrophage proliferation and enhance phagocytosis.

Citations

Nov 3, 2015·International Journal of Biological Macromolecules·Jing XuJing Zhou
Nov 5, 2016·International Journal of Biological Macromolecules·Xiaomeng YuXiaolu Jiang
Nov 21, 2018·Bioinspiration & Biomimetics·Joan Combie, Ebru Toksoy Öner
Oct 19, 2019·Biomolecules·Erin DobrangeWim Van den Ende
Sep 20, 2017·Plant, Cell & Environment·Maxime VersluysWim Van den Ende
Jan 7, 2021·Applied Microbiology and Biotechnology·Rui ChengJianfa Zhang
Aug 29, 2021·Folia Microbiologica·Waqar AhmadMunir Ahmad Anwar

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