Structural Characterization and Immunostimulatory Activity of a Homogeneous Polysaccharide from Sinonovacula constricta

Journal of Agricultural and Food Chemistry
Qingxia YuanXiaoxiong Zeng

Abstract

Sinonovacula constricta has been widely used as a health food and medicine in China, Japan, and Korea. In the present study, a water-soluble polysaccharide fraction (SCP-1) was prepared from S. constricta by enzyme-assisted extraction and purification of chromatography with DEAE-52 cellulose anion-exchange column and Sephadex G-100 size exclusion column. On the basis of the analytical results of high-performance liquid chromatography, Fourier transform-infrared spectroscopy, methylation analysis, and NMR spectroscopy, SCP-1 was found to have an average molecular weight of 15.63 kDa and a linear backbone of (1→4)-linked α-D-Glcp residue with one branch, α-D-Glcp, attached to the main chain by a (1→6) glycosidic bond at every five α-D-Glcp units. Furthermore, it was found that SCP-1 could significantly increase the viability of macrophages, enhance the capability of macrophage phagocytosis, increase the activity of acid phosphatase, and promote the production of nitric oxide, mouse tumor necrosis factor (TNF)-α, mouse interferon (IFN)-γ, and mouse interleukin (IL)-1β. The results suggest that SCP-1 possesses potent immunomodulating effect and may be explored as a potential biological response modifier.

References

May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J F WangS I Chan
Sep 1, 1996·Phytochemistry·C ChauveauL Chavant
Nov 1, 1996·The American Journal of Physiology·J S Beckman, W H Koppenol
Jan 1, 1997·Bioscience, Biotechnology, and Biochemistry·Z L KongP Pan
Jan 1, 1997·Annual Review of Immunology·U BoehmJ C Howard
Jan 26, 1999·Annals of the New York Academy of Sciences·C A Dinarello
Oct 12, 2000·Carbohydrate Research·A V PerepelovY A Knirel
Mar 28, 2001·Science·C R Bertozzi, L L Kiessling
Sep 29, 2001·Nature Immunology·C Bogdan
May 7, 2002·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·T TsukamotoJ Haginaka
Dec 25, 2003·Journal of the American Chemical Society·Ionel Ciucanu, Catherine E Costello
Apr 15, 2004·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Gefei ZhouZuhong Xu
Sep 1, 2004·Carbohydrate Research·Indranil ChakrabortySyed S Islam
Jun 15, 2005·International Immunopharmacology·Guohua ZhaoZongdao Chen
Jan 24, 2006·International Immunopharmacology·Igor A Schepetkin, Mark T Quinn
Jun 13, 2006·The Journal of Biological Chemistry·Vera Carolina B BittencourtEliana Barreto-Bergter
Sep 22, 2007·Journal of Bioscience and Bioengineering·Yunmian HeQuansheng Yao
Dec 11, 2007·International Immunopharmacology·Anwei ChengXueming Xu
Aug 6, 2008·Journal of Agricultural and Food Chemistry·Hirotaka HoshiKenichi Matsunaga
Nov 13, 2009·Nutrition Reviews·Susanna Cunningham-RundlesJeffrey M Perlman
Nov 26, 2009·The Journal of Allergy and Clinical Immunology·Scott P ComminsJohn W Steinke
Sep 2, 2010·International Journal of Biological Macromolecules·Deliang QiaoXiaoxiong Zeng
Mar 3, 2011·Fish & Shellfish Immunology·Chenghua LiTaiwu Li
Mar 21, 2013·International Journal of Biological Macromolecules·Chunlin KeXiaoxiong Zeng
May 2, 2013·Developmental and Comparative Immunology·Donghong NiuJiale Li
Jul 31, 2013·Immunobiology·Maria Rosaria GaldieroSébastien Jaillon
Sep 7, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Changxing JiangQingping Xiong
Apr 29, 2014·Food Chemistry·Zhongfu WangLinjuan Huang
Jun 11, 2014·Critical Reviews in Food Science and Nutrition·Huixian Jiang, Jian Bo Xiao
Oct 16, 2014·Journal of Agricultural and Food Chemistry·Zhuqing DaiXiaoxiong Zeng

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Citations

Feb 19, 2017·International Journal of Biological Macromolecules·Jie LiuJinhua Zhao
Dec 5, 2018·Journal of Food Science·Yan ZengYuanxia Sun
Oct 19, 2017·Critical Reviews in Food Science and Nutrition·Ling Chong WangHao Wu
Jan 3, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Yu ZhangKai-Ping Wang
Dec 6, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yang YangTiehua Zhang
Aug 15, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Yunfei JiXiaobo Li
Jun 30, 2019·International Journal of Biological Macromolecules·Zhurui LiBaoan Song
Jan 29, 2020·International Journal of Biological Macromolecules·Pinyi GaoXuegui Liu
Jan 23, 2021·Journal of Agricultural and Food Chemistry·Guijie ChenZhonghua Liu
Feb 27, 2021·International Journal of Biological Macromolecules·Ming ZhaoHong Xu
Aug 15, 2021·International Journal of Biological Macromolecules·Linlin HuangQuanhong Li
Nov 14, 2017·Journal of Agricultural and Food Chemistry·Qingxia Yuan, Longyan Zhao
Jan 20, 2018·Journal of Agricultural and Food Chemistry·Liyuan YunMin Zhang
Sep 1, 2021·International Journal of Biological Macromolecules·Lin RongTingting Gao

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