Structural characterization and modeling of the Borrelia burgdorferi hybrid histidine kinase Hk1 periplasmic sensor: A system for sensing small molecules associated with tick feeding

Journal of Structural Biology
William J BauerMelissa J Caimano

Abstract

Two-component signal transduction systems are the primary mechanisms by which bacteria perceive and respond to changes in their environment. The Hk1/Rrp1 two-component system (TCS) in Borrelia burgdorferi consists of a hybrid histidine kinase and a response regulator with diguanylate cyclase activity, respectively. Phosphorylated Rrp1 catalyzes the synthesis of c-di-GMP, a second messenger associated with bacterial life-style control networks. Spirochetes lacking either Hk1 or Rrp1 are virulent in mice but destroyed within feeding ticks. Activation of Hk1 by exogenous stimuli represents the seminal event for c-di-GMP signaling. We reasoned that structural characterization of Hk1's sensor would provide insights into the mechanism underlying signal transduction and aid in the identification of activating ligands. The Hk1 sensor is composed of three ligand-binding domains (D1-3), each with homology to periplasmic solute-binding proteins (PBPs) typically associated with ABC transporters. Herein, we determined the structure for D1, the most N-terminal PBP domain. As expected, D1 displays a bilobed Venus Fly Trap-fold. Similar to the prototypical sensor PBPs HK29S from Geobacter sulfurreducens and VFT2 from Bordetella pertussis, apo-...Continue Reading

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Citations

May 6, 2016·Cellular Microbiology·Melissa J CaimanoD Scott Samuels
Feb 9, 2017·Protein Science : a Publication of the Protein Society·R WuP R Pokkuluri
May 10, 2017·ACS Applied Materials & Interfaces·Hui PengXiong Gong
Jul 22, 2018·Frontiers in Cellular and Infection Microbiology·Dan DrecktrahD Scott Samuels
Dec 29, 2020·Parasite Immunology·Jennifer D HelbleLinden T Hu

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