Structural Characterization and Repair Mechanism of Gracilaria lemaneiformis Sulfated Polysaccharides of Different Molecular Weights on Damaged Renal Epithelial Cells

Oxidative Medicine and Cellular Longevity
Da GuoJian-Ming Ouyang

Abstract

Natural Gracilaria lemaneiformis sulfated polysaccharide (GLP0, molecular weight = 622 kDa) was degraded by H2O2 to obtain seven degraded fragments, namely, GLP1, GLP2, GLP3, GLP4, GLP5, GLP6, and GLP7, with molecular weights of 106, 49.6, 10.5, 6.14, 5.06, 3.71, and 2.42 kDa, respectively. FT-IR and NMR results indicated that H2O2 degradation does not change the structure of GLP polysaccharides, whereas the content of the characteristic -OSO3H group (13.46% ± 0.10%) slightly increased than that of the natural polysaccharide (13.07%) after degradation. The repair effects of the polysaccharide fractions on oxalate-induced damaged human kidney proximal tubular epithelial cells (HK-2) were compared. When 60 μg/mL of each polysaccharide was used to repair the damaged HK-2 cells, cell viability increased and the cell morphology was restored, as determined by HE staining. The amount of lactate dehydrogenase released decreased from 16.64% in the injured group to 7.55%-13.87% in the repair groups. The SOD activity increased, and the amount of MDA released decreased. Moreover, the mitochondrial membrane potential evidently increased. All polysaccharide fractions inhibited S phase arrest through the decreased percentage of cells in the S...Continue Reading

References

Jun 14, 2002·Carbohydrate Research·Maria E R DuarteAlberto S Cerezo
Feb 1, 2003·International Journal of Biological Macromolecules·Sutapa MazumderBimalendu Ray
Jun 19, 2013·Food Chemistry·Audrey M FaureLaura Nyström
Jul 20, 2014·Carbohydrate Polymers·Byung Wook Jo, Soo-Kyung Choi
Apr 23, 2015·Journal of Pharmaceutical Sciences·Flavia LaffleurWongsakorn Suchaoin
Sep 2, 2015·Journal of Agricultural and Food Chemistry·Wen-Juan LiMing-Yong Xie
Sep 12, 2015·Natural Product Research·C FaggioM Morabito
Oct 2, 2015·International Journal of Biological Macromolecules·Sadhasivam SudharsanAlagiri Srinivasan
Nov 18, 2015·Carbohydrate Polymers·Mercedes Perez RecaldeMarina Ciancia
Dec 6, 2016·International Journal of Molecular Sciences·Jinzhe HePeilong Sun
Jan 24, 2017·The Journal of Urology·Li Hao Richie XuKhashayar Sakhaee
Feb 19, 2017·World Journal of Urology·Igor SorokinYair Lotan
Jun 21, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Xin-Yuan SunJian-Ming Ouyang
Mar 9, 2018·Advances in Urology·Tilahun Alelign, Beyene Petros

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Citations

May 16, 2020·Oxidative Medicine and Cellular Longevity·Chuang-Ye LiJian-Ming Ouyang
Nov 17, 2020·Frontiers in Physiology·Sakdithep Chaiyarit, Visith Thongboonkerd

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Methods Mentioned

BETA
NMR
Infrared Spectroscopy
Assay
MDA
flow cytometry

Software Mentioned

SPSS

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