PMID: 9539108Apr 16, 1998Paper

Structural characterization and tissue-specific expression of the mouse glucose-6-phosphate dehydrogenase gene

DNA and Cell Biology
D L HodgeL M Salati

Abstract

Glucose-6-phosphate dehydrogenase (G6PD) activity differs among tissues and, in liver, with the dietary state of the mouse. Tissue-specific differences in G6PD activity in adipose tissue, liver, kidney, and heart were associated with similar differences in the amount of G6PD mRNA. Regulation of mRNA amount by dietary fat was only observed in liver. In mice fed a low-fat diet, the relative amounts of G6PD mRNA were 3:1:1:0.38, respectively, in the four tissues. Further, the amount of precursor mRNA for G6PD in liver, kidney, and heart reflected the amount of mature mRNA in these tissues, suggesting differing transcriptional activity. Our S1 nuclease and primer-extension analyses indicated that the same transcriptional start site is used in liver, kidney, and adipose tissue, resulting in a common 5' end of the mRNA in these tissues. Thus, differential regulation is not attributable to alternate promoter usage. A DNase hypersensitivity analysis of the 5' end of the G6PD gene identified three hypersensitive sites (HS): HS 1 and HS 2 were present in all tissues, whereas HS 3 was liver specific. Thus, regulation of G6PD expression involves both dietary and tissue-specific signals that appear to act via different mechanisms.

References

Oct 1, 1979·The Journal of Nutrition·C D Berdanier, D Shubeck
Oct 27, 1975·Biochemical and Biophysical Research Communications·D E Peavy, R J Hansen
Sep 10, 1990·Biochimica Et Biophysica Acta·P LouieD Holten
Feb 1, 1986·Analytical Biochemistry·R M Amasino
May 15, 1973·Biochemical and Biophysical Research Communications·D R Hewish, L A Burgoyne
Jun 11, 1983·Nucleic Acids Research·H P FrittonT Igo-Kemenes
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert
Aug 1, 1994·The Journal of Nutrition·K H Kim, H J Tae
Feb 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R F KletzienL A Foellmi
Jan 1, 1993·DNA Sequence : the Journal of DNA Sequencing and Mapping·M ZolloE Y Chen
Apr 1, 1993·DNA and Cell Biology·H KannoA Yoshida
Aug 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·G Martini, M V Ursini
Aug 15, 1996·Archives of Biochemistry and Biophysics·L P StabileL M Salati
Apr 1, 1958·The American Journal of Physiology·J TEPPERMAN, H M TEPPERMAN

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Citations

May 26, 2001·Annual Review of Nutrition·L M Salati, B Amir-Ahmady
Jul 27, 2010·European Journal of Pharmacology·Elena LarrietaCristina Fernandez-Mejia

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