Structural characterization of a Protein A mimetic peptide dendrimer bound to human IgG

The Journal of Physical Chemistry. B
D MoianiG Fassina

Abstract

Understanding the chemical physical properties of protein binding sites is at the basis of the rational design of protein ligands. The hinge region of the Fc fragment of immunoglobulin G is an important and well characterized protein binding site, known to interact with several natural proteins and synthetic ligands. Here, we report structural evidence that a Staphylococcus aureus Protein A mimetic peptide dendrimer, deduced by a combinatorial approach, binds close to the Cgamma2/Cgamma3 interface of the constant fragment of a human IgG1 molecule, partially hindering the Protein A binding site. The X-ray analysis evidenced a primary binding site located between a terminal Arg residue of the ligand peptidic arm and a hydrophobic protein site consisting of Val308, Leu309, and His310. A molecular dynamic analysis of the model derived from the X-ray structure showed that in water at room temperature the complex is further stabilized by the formation of at least one more contact between a terminal Arg residue of the second arm of the peptide and the carboxylic group of a protein amino acid, such as Glu318, Asp312, or Asp280. It appears thus that stability of the Fc-dendrimer complex is determined by the synergetic formation of multi...Continue Reading

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Citations

Sep 4, 2012·International Journal of Molecular Sciences·Masamichi Nagae, Yoshiki Yamaguchi
Jan 10, 2016·Journal of Chromatography. a·Hong-Fei TongShan-Jing Yao
Nov 1, 2011·Journal of Molecular Recognition : JMR·Francesca DinonGiorgio Fassina
Dec 6, 2011·Journal of Separation Science·Matteo Salvalaglio, Carlo Cavallotti
Aug 19, 2015·Journal of Chromatography. a·Matteo SalvalaglioCarlo Cavallotti
Jul 12, 2017·Bioconjugate Chemistry·Nika Kruljec, Tomaž Bratkovič
Dec 30, 2020·Journal of Pharmaceutical and Biomedical Analysis·Srishti JoshiAnurag S Rathore
Mar 2, 2011·The Journal of Physical Chemistry. B·Diwakar ShuklaBernhardt L Trout
Jul 28, 2016·Bioconjugate Chemistry·Yan ChenFred Jacobson
Jul 20, 2018·Bioconjugate Chemistry·Nika KruljecTomaž Bratkovič
Aug 28, 2021·International Journal of Molecular Sciences·Nunzianna DotiAndrea Caporale

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