Structural characterization of a rice actin gene

Plant Molecular Biology
D McElroyR Wu

Abstract

We have isolated and sequenced a full-length cDNA clone containing information for the rice actin gene RAc1. Transcript terminus mapping and sequence alignment between the RAc1 cDNA clone and a previously isolated RAc1 genomic clone were used to determine the structure of the RAc1 gene. This allowed us to make the first complete structural characterization of a plant actin gene. The analysis revealed the presence of a 5'-noncoding exon, separated by an intron, from the first translated exon of the RAc1 gene. This is one of the few reported cases of a plant gene containing such a 5'-noncoding exon. Sequence comparison between the previously isolated plant actin genes suggests that such an exon may be a common feature of plant actin gene structure. The present study also confirms that the rice actin gene family is composed of at least eight unique members.

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Citations

Nov 1, 1993·Plant Molecular Biology·E E HoodR C Pendleton
Apr 1, 1990·Plant Molecular Biology·K S ReeceR Wu
Dec 1, 1992·Plant Molecular Biology·T TsuchiyaS Ejiri
Aug 1, 1990·Plant Molecular Biology·D McElroyR Wu
Oct 5, 2007·Molecular Biotechnology·George F HeineErich Grotewold
Dec 25, 2008·Molecular Biotechnology·N L Rodríguez-AvilaR Rivera-Madrid
Feb 13, 2007·Plant & Cell Physiology·Naoya HiroseHitoshi Sakakibara
Feb 13, 2001·Plant Physiology·D Van Der StraetenM C Van Montagu
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Peter W. Barlow, Frantisek Baluska
Dec 15, 2010·Bioscience, Biotechnology, and Biochemistry·Chie OtsukaKenji Oda
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·M T Chan, S M Yu
Jun 21, 2014·Plant Molecular Biology·Yuguo XiaoPieter B F Ouwerkerk
Aug 27, 2011·Biochimica Et Biophysica Acta·Maria RegueraEduardo Blumwald
Aug 1, 2008·Journal of Integrative Plant Biology·Ai-Jun SunZhen Zhu
Jun 28, 2006·Yi chuan xue bao = Acta genetica Sinica·Ting-Zhang HuXi-Ping Wang
Oct 21, 1998·The Plant Journal : for Cell and Molecular Biology·M T Chan, S M Yu
Jan 1, 2004·Plant & Cell Physiology·Yutaka SonodaJunji Yamaguchi
Nov 9, 2006·Acta Biochimica Et Biophysica Sinica·Ting-Zhang HuXi-Ping Wang

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