Structural Characterization of Black Widow Spider Dragline Silk Proteins CRP1 and CRP4.

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Mikayla ShanafeltCraig Vierra

Abstract

Spider dragline silk represents a biomaterial with outstanding mechanical properties, possessing high-tensile strength and toughness. In black widows at least eight different proteins have been identified as constituents of dragline silk. These represent major ampullate spidroins MaSp1, MaSp2, MaSp', and several low-molecular weight cysteine-rich protein (CRP) family members, including CRP1, CRP2, and CRP4. Molecular modeling predicts that CRPs contain a cystine slipknot motif, but experimental evidence to support this assertion remains to be reported. To advance scientific knowledge regarding CRP function, we recombinantly expressed and purified CRP1 and CRP4 from bacteria and investigated their secondary structure using circular dichroism (CD) under different chemical and physical conditions. We demonstrate by far-UV CD spectroscopy that these proteins contain similar secondary structure, having substantial amounts of random coil conformation, followed by lower levels of beta sheet, alpha helical and beta turn structures. CRPs are thermally and pH stable; however, treatment with reagents that disrupt disulfide bonds impact their structural conformations. Cross-linking mass spectrometry (XL-MS) data also support computational ...Continue Reading

References

Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M Xu, R V Lewis
Mar 13, 1999·Journal of Molecular Biology·C ScheuflerM Hülsmeyer
May 26, 1999·International Journal of Biological Macromolecules·A M Moore, K Tran
Mar 30, 2001·Nature·F Vollrath, D P Knight
Sep 19, 2002·The Journal of Biological Chemistry·Yves A MullerHeinz Welfle
Aug 29, 2003·Nature·Hyoung-Joon Jin, David L Kaplan
May 11, 2004·Biomacromolecules·Cedric DickoJohn M Kenney
May 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·S RammenseeA R Bausch
Oct 31, 2009·PLoS Computational Biology·Mateusz SikoraMarek Cieplak
May 14, 2010·Nature·Glareh AskariehStefan D Knight
Oct 5, 2010·Journal of Molecular Biology·Michael LandrehTomas Bergman
Jul 11, 2013·Biomacromolecules·Marlene AnderssonAnna Rising
Nov 19, 2013·Nature Communications·Simone SchwarzeHannes Neuweiler
Sep 27, 2014·Biomacromolecules·Thanh PhamCraig Vierra
Oct 30, 2014·Nucleic Acids Research·Wouter G TouwGert Vriend
Sep 21, 2016·International Journal of Molecular Sciences·Camille LarracasCraig A Vierra
Aug 7, 2018·Biomacromolecules·Christopher H BowenFuzhong Zhang
Aug 8, 2018·Proceedings of the National Academy of Sciences of the United States of America·Jun XuAnjiang Tan
Nov 18, 2018·Nature Protocols·Oleg KlykovRichard A Scheltema

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Citations

Sep 18, 2021·Proceedings of the National Academy of Sciences of the United States of America·Tiantian FanZhi Lin

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Datasets Mentioned

BETA
PRJNA242358

Methods Mentioned

BETA
circular dichroism
protein thermal shift
thermal shift
PCR
affinity chromatography
Affinity Purification
protein assay
electrophoresis

Software Mentioned

Proteome Discoverer
CDSSTR
DSSP
CONTIN
Plot2
Excel
Xcalibur
SEQUEST HT
DichroWeb
NetSurfP

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