Abstract
Studies from our laboratory have shown that anti-T12, a mAb which recognizes CD6, is a macrophage-dependent mitogen for human T cells and can augment T cell autoreactivity in vitro. To obtain additional information regarding the potential biological role of CD6 we sought to further characterize its biochemical properties. The CD6 molecule on 125I-surface-labeled T cells and by Western blot analysis was a monomer of mol. wt 130,000 under reducing conditions and mol. wt 117,000 under non-reducing conditions, suggesting the presence of intrachain disulfide bonds. The polypeptide contains a protease sensitive site. In activated T cells, the protein was serine phosphorylated. Analysis of biosynthetically labeled CD6 in the presence of tunicamycin revealed a reduction in mol. wt from 130,000 to 100,000, indicating that the polypeptide is extensively N-glycosylated. The mAb, anti-2H1, had been shown to activate T cells in combination with PMA or the anti-T11(3) mAb but, unlike anti-T12, not in the presence of macrophages alone. The present studies demonstrate by sequential immunoprecipitation that these two mAbs recognize the same polypeptide. However, Western blot analysis and indirect immunofluorescence cross-blocking studies demons...Continue Reading
References
Jul 1, 1978·Immunochemistry·P L EyC R Jenkin
Jul 8, 1975·Biochemical and Biophysical Research Communications·J S Tkacz, O Lampen
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Mar 1, 1986·European Journal of Immunology·U D Staerz, M J Bevan
Jun 1, 1987·European Journal of Immunology·C WalkerW J Pichler
Sep 25, 1987·Cell·D R KaplanT M Roberts
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C E RuddS F Schlossman
Apr 1, 1986·Journal of Reproductive Immunology·S Gupta, E Fanous
Jan 1, 1985·Annual Review of Biochemistry·T Hunter, J A Cooper
Mar 27, 1987·Cell·D R SibleyR J Lefkowitz
Jun 1, 1987·European Journal of Immunology·S CarrelJ C Cerottini
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·F EmmrichK Eichmann
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J A LedbetterP S Rabinovitch
Jun 1, 1986·Neurology·D A HaflerH L Weiner
Sep 1, 1986·The Journal of Experimental Medicine·T R MalekE M Shevach
May 1, 1987·The Journal of Experimental Medicine·S J MentzerS J Burakoff
Sep 1, 1985·The Journal of Experimental Medicine·A MorettaL Moretta
Oct 1, 1985·European Journal of Biochemistry·J VandekerckhoveM Van Montagu
Apr 1, 1985·The Journal of Experimental Medicine·T Hara, S M Fu
May 15, 1985·Analytical Biochemistry·J A SwackS H Wilson
Aug 15, 1970·Nature·U K Laemmli
Dec 1, 1983·Transplantation·R L KirkmanN L Tilney
Apr 1, 1984·Cell·S C MeuerE L Reinherz
Oct 1, 1984·Immunological Reviews·E L ReinherzS F Schlossman
Nov 1, 1984·The Journal of Clinical Investigation·P L Romain, S F Schlossman
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·E L ReinherzS F Schlossman
Oct 1, 1982·Cell·E L ReinherzS F Schlossman
Apr 1, 1980·Cell·E L Reinherz, S F Schlossman
Feb 27, 2007·Oncogene·K A FurgeB T Teh
Citations
Dec 31, 1998·Arthritis and Rheumatism·M C LevesqueD D Patel
Jan 1, 1996·Immunogenetics·O LecomteJ R Parnes
Apr 10, 2013·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Mafalda Pinto, Alexandre M Carmo
Jan 1, 1996·Immunology Letters·A PalJ Stavnezer
Jul 1, 1991·Human Immunology·G Pawelec, H J Bühring
Apr 12, 2002·Veterinary Immunology and Immunopathology·Aric N RogersCynthia L Baldwin
Oct 1, 1991·The Journal of Experimental Medicine·A AruffoB Seed
Jan 1, 1993·The Journal of Experimental Medicine·S WeeA Aruffo
Apr 1, 1995·The Journal of Experimental Medicine·D D PatelB F Haynes
Jun 1, 1995·The Journal of Experimental Medicine·M A BowenU Francke
Aug 19, 2008·Hybridoma·Ruby AlonsoEnrique Montero
Feb 12, 2011·Briefings in Functional Genomics·Anu KemppinenAlastair Compston
Apr 28, 2009·BMC Genomics·Carolyn T A Herzig, Cynthia L Baldwin
May 29, 2013·FEBS Letters·Lizette BonetFrancisco Lozano
Feb 1, 1994·Research in Immunology·D D Patel, B F Haynes
Jan 1, 2012·Results in Immunology·Pedro C RodriguezPatricia Hernandez
Jan 24, 2007·PLoS Pathogens·Eric BarteeKlaus Früh
May 4, 2010·Experimental Parasitology·Neide Maria SilvaJosé Roberto Mineo
Sep 8, 2005·Veterinary Immunology and Immunopathology·Aric N RogersCynthia L Baldwin
Jul 19, 1996·The Journal of Biological Chemistry·M A BowenA Aruffo
Oct 1, 1995·European Journal of Immunology·W H RobinsonJ R Parnes
Dec 12, 1997·European Journal of Immunology·J KobargM A Bowen
Dec 11, 2002·The Journal of Biological Chemistry·Idoia GimferrerFrancisco Lozano
Jul 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Idoia GimferrerFrancisco Lozano
Jul 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anna IbáñezFrancisco Lozano
Aug 4, 1995·The Journal of Biological Chemistry·G S WhitneyA Aruffo
Jun 26, 1998·Immunology·L M OsorioS C Chow
Nov 6, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mohammad K SaifullahNora G Singer
Oct 26, 2018·Frontiers in Immunology·Marjolein B M MeddensAlessandra Cambi
May 19, 2009·Molecular Immunology·Mònica ArmanFrancisco Lozano
Nov 9, 1990·Science·Y FurukawaJ D Griffin