Structural characterization of diC14-amidine, a pH-sensitive cationic lipid used for transfection

Chemistry and Physics of Lipids
Carlos R BenattiM Teresa Lamy

Abstract

The structure of N-t-butyl-N'-tetradecyl-3-tetradecylaminopropionamidine (diC(14)-amidine) cationic vesicles, used for transfection, was investigated at different pH values and ionic strengths, through the analysis of the electron spin resonance (ESR) spectra of spin labels. Phospholipid derivatives, spin labeled at the 5th and 16th C-atoms along the hydrocarbon chain, incorporated in diC(14)-amidine bilayers, show that the bilayer structure is highly sensitive to the pH value of the medium, due to the two titratable groups present in the amphiphile. Compared with samples at higher pH values, the double charged diC(14)-amidine at pH 3 presents a rather non-organized bilayer gel phase, and a much lower gel-fluid temperature transition, in accord with a strong headgroup electrostatic repulsion. In addition, the structure was found to be highly dependent on the ionic strength of the medium. However, pH 3 diC(14)-amidine bilayer, in the fluid phase, was found to be slightly more closely packed than those at pH 7.4 or 9.0, which are less charged. Parallel to that, the larger isotropic hyperfine splitting measured for nitroxides in the center of the pH 3 diC(14)-amidine bilayer suggests a higher membrane polarity for the highly charg...Continue Reading

References

Dec 15, 1978·Biochimica Et Biophysica Acta·S SchreierI C Smith
Aug 30, 1991·Biochemical and Biophysical Research Communications·X Gao, L Huang
Jan 26, 1989·Nature·P L Felgner, G M Ringold
Feb 12, 1996·FEBS Letters·A El OuahabiJ M Ruysschaert
May 30, 1997·Chemistry and Physics of Lipids·T PaukkuP K Kinnunen
Mar 29, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·R RameshJ A Roth
Jul 18, 2001·Chemistry and Physics of Lipids·C R BenattiM T Lamy-Freund
Feb 25, 2003·Chemistry and Physics of Lipids·M Teresa Lamy-Freund, Karin A Riske

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Citations

Jun 23, 2006·Journal of Fluorescence·Cíntia C De Vequi-SuplicyM Teresa Lamy
Apr 22, 2011·Expert Review of Vaccines·Dennis ChristensenElse Marie Agger
Oct 8, 2011·International Journal of Pharmaceutics·Mamta KapoorSiddhesh D Patil
Oct 16, 2014·Biochimica Et Biophysica Acta·Julio H K RozenfeldM Teresa Lamy
Mar 6, 2007·Colloids and Surfaces. B, Biointerfaces·Ahmad AljaberiMichalakis Savva
Mar 8, 2006·Colloids and Surfaces. B, Biointerfaces·Sung-Hee ParkSung-Sik Han
Feb 24, 2009·Biochimica Et Biophysica Acta·Carlos R BenattiM Teresa Lamy
Feb 16, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Tiago R OliveiraCaroline Lonez
Aug 4, 2009·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·T R OliveiraT B Paiva
Jul 31, 2009·Chemical Communications : Chem Comm·Santanu Bhattacharya, Avinash Bajaj
Aug 25, 2017·Biophysics Reviews·Julio H K RozenfeldM Teresa Lamy

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