Structural characterization of five post-translationally modified isomorphs of a novel putative delta-conotoxin from the vermivorous snail Conus delessertii from the Mexican Caribbean Sea

Peptides
Manuel B AguilarEdgar P Heimer de la Cotera

Abstract

A novel peptide, de7b, was isolated from the venom of Conus delessertii, a worm-hunting species collected in the Caribbean Sea off the Yucatan Peninsula. Its primary structure was determined by automated Edman degradation and confirmed by mass spectrometry: it contains 28 amino acids, including six Cys residues. Peptide de7b is the second, O-conotoxin-like peptide isolated from the venom of this species, and it exists in different post-translationally modified isomorphs, some of which contain gamma-carboxy-glutamate (gamma) and/or 4-hydroxy-proline (O) at positions 4, 7, and/or 14. Its primary structure is DCI(P/O)GG(E/gamma)NCDVFR(O/P)YRCCSGYCILLLCA, with molecular masses varying from 3078.6 to 3154.6Da, depending on the number and kind of modified amino acid residues. Peptide de7b shows significant sequence identity with several O-conotoxins purified and biologically characterized from molluscivorous and piscivorous cone snails of the Indo-Pacific region, the tropical Atlantic and Eastern Pacific Oceans, especially with the delta-conotoxins but also with the omega-conotoxins from molluscivorous species, which suggests that it might affect voltage-gated Na(+) or Ca(2+)channels. Peptide de7b has 32% sequence identity with putat...Continue Reading

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Citations

Mar 10, 2010·Toxicon : Official Journal of the International Society on Toxinology·Quentin KaasDavid J Craik
Dec 22, 2010·Toxicon : Official Journal of the International Society on Toxinology·C A ElligerW F Gilly
Dec 15, 2010·Natural Product Reports·John W BluntMichèle R Prinsep
Jan 23, 2020·Marine Drugs·Dawrin Pech-PuchCarlos Jiménez
Aug 9, 2020·Marine Drugs·Anna M L KlompenSérgio N Stampar

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