Structural characterization of hemicellulose released from corn cob in continuous flow type hydrothermal reactor

Journal of Bioscience and Bioengineering
Tsutomu AraiYoshihiko Amano

Abstract

Hydrothermal reaction is known to be one of the most efficient procedures to extract hemicelluloses from lignocellulosic biomass. We investigated the molecular structure of xylooligosaccharides released from corn cob in a continuous flow type hydrothermal reactor designed in our group. The fraction precipitable from the extract with four volumes of ethanol was examined by 1H-NMR spectroscopy and MALDI-TOF MS before and after enzymatic treatment with different purified enzymes. The released water-soluble hemicellulose was found to correspond to a mixture of wide degree of polymerization range of acetylarabinoglucuronoxylan fragments (further as corn cob xylan abbreviated CX). Analysis of enzymatic hydrolyzates of CX with an acetylxylan esterase, GH3 β-xylosidase, GH10 and GH11 xylanases revealed that the main chain contains unsubstituted regions mixed with regions of xylopyranosyl residues partially acetylated and occasionally substituted by 4-O-methyl-d-glucuronic acid and arabinofuranose esterified with ferulic or coumaric acid. Single 2- and 3-O-acetylation was accompanied by 2,3-di-O-acetylation and 3-O-acetylation of Xylp residues substituted with MeGlcA. Most of the non-esterified arabinofuranose side residues were lost du...Continue Reading

Citations

Jan 11, 2020·Frontiers in Chemistry·Aya Zoghlami, Gabriel Paës
Jan 21, 2021·Annual Review of Food Science and Technology·Shang LinAnne S Meyer
Jul 10, 2021·Frontiers in Bioengineering and Biotechnology·Ling-Zhi HuangChuanling Si

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