Structural characterization of lipopeptide biomarkers isolated from Bacillus globigii

Journal of Mass Spectrometry : JMS
Bruce H WilliamsCatherine Fenselau

Abstract

Spectra obtained using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry of Bacillus globigii (Bacillus subtilis niger) spores, vegetative cells and the culture supernatant show a cluster of biomarkers centered at a molecular mass of 1478 Da. Three biomarkers were isolated from the cell-free culture supernatant by solid-phase extraction and reversed-phase high-performance liquid chromatography, and characterized using various kinds of mass spectrometry. A Fourier transform mass spectrometer with a MALDI source was used to determine the monoisotopic protonated masses at 1463.8, 1477.8, and 1505.8 Da in order of elution. The mass differences of 14 and 28 Da suggest that they are homologous molecules. Alkaline hydrolysis of each species showed that it contained a lactone linkage. Strong acid hydrolysis released a fatty acid from an amide bond, consistent with a lipopeptide. A quadrupole time-of-flight instrument with a nanospray source was used to sequence the hydrolyzed forms of the three biomarkers. The cyclic lipopeptides were found to have amino acid sequences identical with those in fengycins and plipastatins, antimicrobial compounds with phospholipase inhibitor activity, previously identified in related s...Continue Reading

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Citations

Jul 6, 2007·Applied Microbiology and Biotechnology·J WangJ Yao
Aug 1, 2012·Journal of the American Society for Mass Spectrometry·Khyati V PathakPadmanabhan Balaram
Apr 10, 2013·Journal of the American Society for Mass Spectrometry·Plamen A DemirevAndrew B Feldman
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Oct 19, 2004·Rapid Communications in Mass Spectrometry : RCM·Plamen A Demirev
Oct 14, 2004·Journal of Applied Microbiology·P I KimY-T Chi
Aug 23, 2008·Journal of Mass Spectrometry : JMS·Plamen A Demirev, Catherine Fenselau
Jul 14, 2010·Journal of Mass Spectrometry : JMS·Ylenia PecciGianna Allegrone
Aug 5, 2016·Rapid Communications in Mass Spectrometry : RCM·Gabriella FavaroBarbara Baldan
Jul 12, 2002·Journal of Mass Spectrometry : JMS
Mar 7, 2021·International Journal of Molecular Sciences·Ana B MoldesJosé M Cruz

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