PMID: 9182997May 1, 1997Paper

Structural characterization of N-linked oligosaccharides from cellobiohydrolase I secreted by the filamentous fungus Trichoderma reesei RUTC 30

European Journal of Biochemistry
M MarasR Contreras

Abstract

We have characterized the primary structures of the predominant N-linked oligosaccharides on cellobiohydrolase I from the filamentous fungus Trichoderma reesei RUTC30. Different enzymatic and chromatographic techniques were used to analyze six oligosaccharides. The combined data showed that the fungal carbohydrates have a core structure that is identical to the mammalian N-linked core. In the bulk of the N-glycans, the alpha-1,3 arm is extended with two mannoses and a glucose, suggesting incomplete processing of the oligosaccharides in the endoplasmic reticulum. The alpha-1,6 arm shows a remarkable heterogeneity: in addition to alpha-1,2-Man and alpha-1,6-Man, the presence of a terminal mannose alpha-1,6-phosphodiester was observed. This latter substituent has not been characterized before on mannosidase-processed N-glycan and its function and synthesis pathway are entirely unknown. The predominant N-glycans on cellobiohydrolase I can be represented as follows: GlcMan8GlcNAc2, GlcMan7GlcNAc2, Man7GlcNAc2, ManPGlcMan7GlcNAc2, GlcMan5GlcNAc2 and Man5GlcNAc2.

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Citations

Apr 28, 2010·Applied Microbiology and Biotechnology·Orquídea RibeiroMerja Penttilä
Jun 30, 2010·Applied Microbiology and Biotechnology·Karen De PourcqNico Callewaert
Jun 27, 2009·The Journal of Microbiology·Alexandre Favarin SomeraJoão Atílio Jorge
Jun 4, 2002·Glycobiology·Mathew J HarrisonK M Helena Nevalainen
May 28, 2008·Glycobiology·Nandan DeshpandeHelena Nevalainen
Mar 20, 2004·European Journal of Biochemistry·Torny ErikssonHarry Brumer
Dec 18, 2007·Applied and Environmental Microbiology·Elke KainzJoseph Strauss
May 13, 2008·Biotechnology for Biofuels·Tina JeohWilliam S Adney
Dec 18, 2009·FEMS Microbiology Letters·Ingeborg StalsKathleen Piens
Nov 17, 2007·Carbohydrate Research·Alexander V GusakovBoris B Ustinov
Oct 15, 2011·Microbiology·Robyn Peterson, Helena Nevalainen
Apr 18, 2002·Canadian Journal of Microbiology·Marko J NykänenAnita Mikkonen
May 16, 1998·Biochemical and Biophysical Research Communications·E V EneyskayaK N Neustroev
Jan 30, 2015·Chemical Reviews·Christina M PayneGregg T Beckham

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