Structural characterization of Salmonella typhimurium YeaZ, an M22 O-sialoglycoprotein endopeptidase homolog.

Proteins
C E NicholsD K Stammers

Abstract

The Salmonella typhimurium "yeaZ" gene (StyeaZ) encodes an essential protein of unknown function (StYeaZ), which has previously been annotated as a putative homolog of the Pasteurella haemolytica M22 O-sialoglycoprotein endopeptidase Gcp. YeaZ has also recently been reported as the first example of an RPF from a gram-negative bacterial species. To further characterize the properties of StYeaZ and the widely occurring MK-M22 family, we describe the purification, biochemical analysis, crystallization, and structure determination of StYeaZ. The crystal structure of StYeaZ reveals a classic two-lobed actin-like fold with structural features consistent with nucleotide binding. However, microcalorimetry experiments indicated that StYeaZ neither binds polyphosphates nor a wide range of nucleotides. Additionally, biochemical assays show that YeaZ is not an active O-sialoglycoprotein endopeptidase, consistent with the lack of the critical zinc binding motif. We present a detailed comparison of YeaZ with available structural homologs, the first reported structural analysis of an MK-M22 family member. The analysis indicates that StYeaZ has an unusual orientation of the A and B lobes which may require substantial relative movement or inter...Continue Reading

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Citations

Nov 17, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·C E NicholsD K Stammers
Oct 15, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Qingping XuIan A Wilson
Oct 15, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Eugene V Koonin
May 6, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Inci AydinAnna Roujeinikova
Apr 21, 2009·Journal of Bacteriology·Jennifer I HandfordTracy Palmer
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Aug 19, 2014·Frontiers in Public Health·Thandavarayan RamamurthySumio Shinoda
Jan 9, 2018·Nucleic Acids Research·Amit LuthraManal A Swairjo
Mar 2, 2012·The Journal of Biological Chemistry·Christopher DeutschDirk Iwata-Reuyl
Feb 13, 2020·Nucleic Acids Research·Jing-Bo ZhouXiao-Long Zhou
Oct 7, 2021·Nucleic Acids Research·Jonah Beenstock, Frank Sicheri

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