Structural characterization of surface glycans from Clostridium difficile

Carbohydrate Research
Christopher W ReidJ R Brisson

Abstract

Whole-cell high-resolution magic angle spinning (HR-MAS) NMR was employed to survey the surface polysaccharides of a group of clinical and environmental isolates of Clostridium difficile. Results indicated that a highly conserved surface polysaccharide profile among all strains studied. Multiple additional peaks in the anomeric region were also observed which prompted further investigation. Structural characterization of the isolated surface polysaccharides from two strains confirmed the presence of the conserved water soluble polysaccharide originally described by Ganeshapillai et al. which was composed of a hexaglycosyl phosphate repeat consisting of [→6)-β-D-Glcp-(1-3)-β-D-GalpNAc-(1-4)-α-D-Glcp-(1-4)-[β-D-Glcp(1-3]-β-D-GalpNAc-(1-3)-α-D-Manp-(1-P→]. In addition, analysis of phenol soluble polysaccharides revealed a similarly conserved lipoteichoic acid (LTA) which could be detected on whole cells by HR-MAS NMR. Conventional NMR and mass spectrometry analysis indicated that the structure of this LTA consisted of the repeat unit [→6)-α-D-GlcpNAc-(1-3)-[→P-6]-α-D-GlcpNAc-(1-2)-D-GroA] where GroA is glyceric acid. The repeating units were linked by a phosphodiester bridge between C-6 of the two GlcNAc residues (6-P-6). A minor ...Continue Reading

References

Jul 22, 1998·Extremophiles : Life Under Extreme Conditions·R H DoiA Ibrahim
Apr 12, 2002·The Lancet Infectious Diseases·Isaac Ginsburg
Jul 18, 2009·International Journal of Antimicrobial Agents·Hanna Pituch
Aug 12, 2009·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Jenni AntikainenPäivi Tissari
Sep 18, 2009·Journal of Lipid Research·Ken'ichi Ichihara, Yumeto Fukubayashi

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Citations

May 14, 2014·Annual Review of Microbiology·Matthew G Percy, Angelika Gründling
Mar 29, 2014·Molecular Microbiology·Eiji TamaiShigehiro Kamitori
Mar 19, 2014·Human Vaccines & Immunotherapeutics·Rosanna LeuzziMaria Scarselli
Jan 21, 2014·Chemistry & Biology·Chakkumkal AnishPeter H Seeberger
Feb 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Stefan G RichterDominique Missiakas
Jun 18, 2016·Microbial Biotechnology·Joseph A KirkRobert P Fagan
Aug 9, 2016·Archives of Pharmacal Research·Seok-Seong KangSeung Hyun Han
Apr 9, 2013·Expert Review of Vaccines·Mario A MonteiroHerbert Chow
Dec 19, 2016·Chemical Society Reviews·Daan van der EsJeroen D C Codée
Mar 17, 2018·FEMS Microbiology Reviews·Francesca MicoliRoberto Adamo
Aug 30, 2014·Chemistry : a European Journal·Wouter F J HogendorfChristian Marcus Pedersen
Jan 15, 2014·Journal of Bacteriology·Olaf Schneewind, Dominique Missiakas
May 16, 2019·Infection and Immunity·Elodie CuenotIsabelle Martin-Verstraete
Jul 10, 2013·Chemical Communications : Chem Comm·Christopher E MartinPeter H Seeberger
May 18, 2019·NPJ Biofilms and Microbiomes·Thomas DuboisBruno Dupuy
Sep 6, 2018·Current Medicinal Chemistry·Gerald Larrouy-Maumus
Sep 2, 2020·Journal of Bacteriology·Gabriela M KausCraig D Ellermeier
May 10, 2020·NPJ Biofilms and Microbiomes·Thomas DuboisBruno Dupuy
Nov 7, 2019·ACS Chemical Biology·Felix BroeckerPeter H Seeberger

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