Structural characterization of the acid-degraded secondary cell wall polymer of Geobacillus stearothermophilus PV72/p2

Carbohydrate Research
Bent O PetersenP Kosma

Abstract

The secondary cell wall polymer (SCWP) from Geobacillus stearothermophilus PV72/p2, which is involved in the anchoring of the surface-layer protein to the bacterial cell wall layer, is composed of 2-amino-2-deoxy- and 2-acetamido-2-deoxy-D-glucose, 2-acetamido-2-deoxy-D-mannose, and 2-acetamido-2-deoxy-D-mannuronic acid. The primary structure of the acid-degraded polysaccharide--liberated by HF-treatment from the cell wall--was determined by high-field NMR spectroscopy and mass spectrometry using N-acetylated and hydrolyzed polysaccharide derivatives as well as Smith-degradation. The polysaccharide was shown to consist of a tetrasaccharide repeating unit containing a pyruvic acid acetal at a side-chain 2-acetamido-2-deoxy-alpha-D-mannopyranosyl residue. Substoichiometric substitutions of the repeating unit were observed concerning the degree of N-acetylation of glucosamine residues and the presence of side-chain linked 2-acetamido-2-deoxy-beta-D-glucopyranosyl units: [Formula: see text].

References


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Citations

Jun 22, 2011·The Journal of Biological Chemistry·Christine HorejsDietmar Pum
Sep 14, 2011·The Journal of Biological Chemistry·Gerald PoschChristina Schäffer
Oct 24, 2009·Natural Product Research·Ji ZhangYun-Pu Wang
Jan 28, 2009·Acta Crystallographica. Section D, Biological Crystallography·Thomas Lütteke
Oct 18, 2011·Biochemistry. Biokhimii︠a︡·E M Tul'skayaL I Evtushenko
Feb 4, 2014·FEMS Microbiology Reviews·Uwe B SleytrDietmar Pum
Aug 28, 2016·FEMS Microbiology Reviews·Christina Schäffer, Paul Messner
Apr 23, 2013·Small·Mehmet H UcisikUwe B Sleytr

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