PMID: 2500660Jul 1, 1989Paper

Structural characterization of the alpha-glycerol-3-phosphate dehydrogenase-encoding gene of Drosophila melanogaster

Proceedings of the National Academy of Sciences of the United States of America
L von KalmD T Sullivan

Abstract

In Drosophila, multiple isoforms of alpha-glycerol-3-phosphate dehydrogenase (sn-glycerol-3-phosphate: NAD+ 2-oxidoreductase, EC 1.1.1.8) are produced in a tissue- and stage-specific manner. To understand the underlying molecular basis of these isoforms, we have sequenced a 5.8-kilobase region of the Drosophila genome that contains the entire Gpdh locus. Primer-extension and RNase protection assays show that the gene consists of eight exons and has a single transcription-start point. RNase protection mapping and comparison of the genomic sequence from three different cDNA clones reveal that three protein isoforms of glycerol-3-phosphate dehydrogenase are produced by alternative processing of 3' exons. Two of the isoforms differ from the third by the addition of either three or ten amino acids to their C-terminal ends. Transcripts corresponding to two of the isoforms are expressed during both larval and adult stages, while the third isoform is produced only in adults.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson

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Citations

Apr 1, 1991·Molecular & General Genetics : MGG·D C KnippleR J MacIntyre
Nov 1, 1992·Molecular & General Genetics : MGG·L Roselli-RehfussD T Sullivan
Nov 1, 1991·Molecular & General Genetics : MGG·R L Shaw-LeeD T Sullivan
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·T M WilanowskiJ E Symonds
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·F J AyalaJ Kwiatowski
Oct 6, 1997·Molecular Biology of the Cell·K WojtasD Sullivan
Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·F J Ayala
Jan 1, 1997·Journal of Molecular Evolution·J KwiatowskiF J Ayala
Sep 13, 2001·Proceedings of the National Academy of Sciences of the United States of America·F Rodríguez-TrellesF J Ayala
Apr 22, 1996·Proceedings. Biological Sciences·R S Wells
May 21, 2003·The Journal of Experimental Biology·David T SullivanL Ramizel
Aug 21, 2013·The Journal of Experimental Biology·Youngjin Park, Yonggyun Kim

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