PMID: 11779506Jan 10, 2002

Structural characterization of the intramolecular interaction between the SH3 and guanylate kinase domains of PSD-95

Molecular Cell
G A TavaresA T Brunger

Abstract

PSD-95/SAP90 is a member of the MAGUK superfamily. In excitatory synapses, PSD-95 clusters receptors and ion channels at specific sites in the postsynaptic membrane and organizes downstream signaling and cytoskeletal molecules. We have determined the crystal structures of the apo and GMP-bound forms to 2.3 and 2.0 A resolutions, respectively, of a fragment containing the SH3, HOOK, and guanylate kinase (GK) domains of PSD-95. We observe an intramolecular interaction between the SH3 and GK domains involving the formation of a beta sheet including residues N- and C-terminal to the GK domain. Based on amino acid conservation and mutational data available in the literature, we propose that this intramolecular interaction is a common feature among MAGUK proteins.

References

Feb 21, 2003·Current Opinion in Neurobiology·Aaron W McGee, David S Bredt
Sep 23, 2009·Biochemistry·Jana MarcetteKenneth E Prehoda
Sep 21, 2004·Nature Reviews. Neuroscience·Eunjoon Kim, Morgan Sheng
Jan 21, 2009·Nature Reviews. Neuroscience·Wei Feng, Mingjie Zhang
Jan 16, 2007·Nature Structural & Molecular Biology·Michael L ReeseVolker Dötsch
Oct 13, 2011·Proceedings of the National Academy of Sciences of the United States of America·Christopher A JohnstonKenneth E Prehoda
Sep 29, 2012·Proceedings of the National Academy of Sciences of the United States of America·James J McCannMark E Bowen
Apr 20, 2011·The Journal of Biological Chemistry·Erick Miranda-LafertePatricia Hidalgo
Nov 23, 2005·The Journal of Experimental Medicine·Hisaaki ShinoharaTomohiro Kurosaki
Sep 28, 2005·The Journal of General Physiology·Shoji X TakahashiHenry M Colecraft
Dec 22, 2006·Molecular Biology of the Cell·Qian WangBen Margolis
Dec 22, 2006·Molecular Biology of the Cell·Alan S FanningJames M Anderson
Aug 6, 2010·Cold Spring Harbor Perspectives in Biology·Margot ThomeStephan Hailfinger
May 26, 2009·Acta Crystallographica. Section D, Biological Crystallography·Thomas C TerwilligerLi Wei Hung
Dec 17, 2009·Molecular and Cellular Biology·Miguel E Moreno-GarcíaDavid J Rawlings
Jan 14, 2011·American Journal of Physiology. Renal Physiology·Jay Pieczynski, Ben Margolis
Apr 4, 2009·Physiological Reviews·Shuiping DaiJohannes W Hell
Mar 13, 2010·Journal of Biomedicine & Biotechnology·H BauerH C Bauer
Aug 7, 2007·BMC Evolutionary Biology·Aartjan J W te VelthuisChristoph P Bagowski
Nov 14, 2006·PLoS Computational Biology·Dmitry KorkinAndrej Sali
May 1, 2012·PloS One·Christopher A JohnstonKenneth E Prehoda
Jul 19, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Eric I LinWilliam N Green
Jan 21, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Eric S SweetBonnie L Firestein
Mar 12, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Karen Perez de ArceAlejandra R Alvarez
Aug 19, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Michael J BianchettaMaria A Morabito
Feb 12, 2014·Cell Research·Jinwei ZhuMingjie Zhang
Apr 22, 2004·Proceedings of the National Academy of Sciences of the United States of America·Shoji X TakahashiHenry M Colecraft
Sep 17, 2011·Molecular and Cellular Neurosciences·Harold D MacGillavryThomas A Blanpied
Aug 12, 2009·Experimental Cell Research·Martin BaumgartnerKarin Moelling
Oct 30, 2014·Protein Science : a Publication of the Protein Society·Jimin Wang
Sep 24, 2014·Cell Communication and Signaling : CCS·Gisela SchimmackDaniel Krappmann
Sep 24, 2008·Protein Science : a Publication of the Protein Society·Ingo PaarmannManfred Konrad
Sep 23, 2006·The EMBO Journal·Lorraine V KaliaMichael W Salter

Citations

Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Nov 1, 1989·Analytical Biochemistry·S C Gill, P H von Hippel
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·R A LueA H Chishti
Apr 1, 1996·Current Biology : CB·J M Anderson
Feb 13, 1997·Nature·F SicheriJ Kuriyan
May 2, 1997·The Journal of Biological Chemistry·M TakeuchiY Takai
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·Paul D AdamsA T Brunger
Feb 12, 1998·Science·T Pawson, J D Scott
Jan 14, 1998·Neuron·E B Ziff
Apr 2, 1998·European Journal of Biochemistry·S KuhlendahlC C Garner
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·N S PannuRandy J Read
Jun 4, 1999·Trends in Cell Biology·M Colledge, J D Scott
Jun 11, 1999·The Journal of Biological Chemistry·A W McGee, David S Bredt
Aug 17, 1999·Current Opinion in Cell Biology·Alan S Fanning, J M Anderson
Sep 18, 1999·Acta Crystallographica. Section D, Biological Crystallography·Ralf W Grosse-Kunstleve, A T Brunger
Oct 12, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S D DimitratosP J Bryant
May 18, 2000·Nature Neuroscience·C LüscherD Muller
Jun 9, 2000·Annual Review of Physiology·Morgan Sheng, D T Pak
Jul 6, 2000·FEBS Letters·K Dodge, J D Scott
Nov 4, 2000·The EMBO Journal·H WuC C Garner
Mar 17, 2001·Nature Reviews. Neuroscience·R H Scannevin, R L Huganir
May 31, 2001·The Journal of Cell Biology·S TomitaDavid S Bredt
Jan 1, 1996·Acta Crystallographica. Section D, Biological Crystallography·J P Abrahams, A G Leslie
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

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