PMID: 9440048Jan 24, 1998Paper

Structural characterization of the molten globule and native states of ovalbumin: a 1H NMR study

The Journal of Peptide Research : Official Journal of the American Peptide Society
M SogamiN Nagai

Abstract

Molecular characteristics of ovalbumin (OVA) in the acidic (pD 3.08, the E-form) and neutral [pD 7.29, the N-form (native form)] regions were studied by measuring effective radii, 1H NMR spectra, spin-echo 1H NMR spectra and cross-relaxation times (TIS) from irradiated to observed protein protons which are particularly sensitive for detection of the mobile segments and/or structural looseness in proteins. 1H NMR spectra did not show significant differences between the N- and E-forms except for the spectral lines in the CH3, epsilon CH2 and aromatic regions. Effective radii and TIS values for main- and side-chains showed 1.08 and 1.5- to 2.0-fold increases on going from the N- to E-forms, respectively. The elongation of TIS values might indicate the appearance of the fluctuating tertiary structure in the E-form. Molecular characteristics of the E-form, inferred from reported far ultraviolet-circular dichroism (UV-CD) spectra in the peptide region, near UV-CD spectra in the aromatic region [Koseki et al. (1988) J. Biochem. 103, 425-430], effective radii and especially elongation of TIS values might indicate that the E-form could be in the molten globule state. The onset of denaturation of OVA using TIS measurements was also studied.

References

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Citations

Jan 30, 1999·The Journal of Peptide Research : Official Journal of the American Peptide Society·S Era, M Sogami
Oct 12, 2005·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Shigeru MatsushimaYasutomi Kinosada
May 13, 2010·Korean Journal of Radiology : Official Journal of the Korean Radiological Society·Hideyuki NishiofukuKimihiko Kichikawa
Mar 17, 2010·NMR in Biomedicine·Koji NakamuraYasutomi Kinosada
Jun 29, 2004·Journal of Magnetic Resonance Imaging : JMRI·Sachiko YuenTsunehiko Nishimura
Feb 11, 2003·NMR in Biomedicine·Masaru SogamiKazuo Kato
Jul 21, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·N F Martins, M M Santoro
Nov 6, 2008·International Journal of Biological Macromolecules·Seiichi EraKazuo Kuwata
Feb 28, 2008·The Journal of Physical Chemistry. B·Patrizia AndreozziCamillo La Mesa
Feb 13, 2007·Biomacromolecules·Alessandro CiurleoCamillo La Mesa

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