Structural characterization of the monolayer-multilayer transition in a pulmonary surfactant model: IR studies of films transferred at continuously varying surface pressures

Langmuir : the ACS Journal of Surfaces and Colloids
Guangru MaoRichard Mendelsohn

Abstract

The four-component system acyl chain perdeuterated 1,2-dipalmitoylphosphatidylcholine (DPPC)/1,2-dipalmitoylphosphatidylglycerol/ (DPPG)/pulmonary surfactant protein SP-C/cholesterol provides a useful model for in vitro biophysical studies of the reversible monolayer to multilayer transition that occurs during compression <--> expansion cycles in the lung. Monolayer films of this mixture (with chain perdeuterated DPPC-d62) at the air/water interface have been transferred to solid substrates under conditions of continuously varying surface pressure, an approach termed COVASP (continuously varying surface pressures) (Langmuir 2007, 23, 4958). The thermodynamic properties of the Langmuir films have been examined with pressure-area isotherms, while the molecular properties of the film constituents in the transferred films in the monolayer and multilayer phases have been examined with IR spectroscopy. Quantitative intensity measurements of the DPPC-d62, DPPG, and SP-C components in each phase reveal that the DPPG and SP-C constituents are relatively enriched in the multilayer compared with the DPPC-d62, although all three species are present in both phases. Some molecular structure information is available from the surface-pressure-...Continue Reading

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Citations

Jun 9, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Pedro H B AokiCarlos J L Constantino
Feb 17, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Robert K Thomas, Jeffrey Penfold
Dec 31, 2011·Biochimica Et Biophysica Acta·Eleonora KeatingRuud A W Veldhuizen
Feb 21, 2009·Faraday Discussions·Avishek GhoshMischa Bonn
Sep 28, 2014·Chemistry and Physics of Lipids·Elisa Parra, Jesús Pérez-Gil
Jun 17, 2010·Physiology·Jesús Perez-Gil, Timothy E Weaver
Jul 16, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Pedro H B AokiCarlos J L Constantino
Mar 27, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Philip ShushkovAlia Tadjer
Mar 27, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Stanislav TzvetanovAlia Tadjer
Mar 23, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Guangru MaoRussel M Walters
Oct 18, 2011·The Journal of Physical Chemistry. B·Nirod Kumar Sarangi, Archita Patnaik
Jun 26, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Fernando Fajardo-RojasJoseph Samaniuk

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