PMID: 9545492May 8, 1998Paper

Structural characterization of the mouse long-chain acyl-CoA dehydrogenase gene and 5' regulatory region

Mammalian Genome : Official Journal of the International Mammalian Genome Society
D M KurtzP A Wood

Abstract

Long-chain acyl-CoA dehydrogenase (LCAD) is one of four enzymes involved in the initial step of mitochondrial beta-oxidation of straight-chain fatty acids. It is a member of the acyl-CoA dehydrogenase (Acad or ACAD) gene family of enzymes, which also includes very-long-chain (VLCAD), medium-chain (MCAD), and short-chain (SCAD) acyl-CoA dehydrogenases. These enzymes all have similar activity but differ only in the chain length specificity for their substrate. Mitochondrial beta-oxidation provides an important source of energy especially during times of fasting. In order to understand the role of LCAD in this pathway, we have cloned and characterized the entire mouse (Mus musculus) gene encoding LCAD (Acadl). Acadl is a single-copy, nuclear encoded gene approximately 35 kb in size. We have sequenced the entire coding region, all intron/exon boundaries, 1.7 kb of its 5' regulatory region, and mapped the transcription start site. The gene contains 11 coding exons ranging in size from 67 bp to 275 bp, interrupted by 10 introns ranging in size from 1.0 kb to 6.6 kb in size. The Acadl 5' regulatory region, like other members of the Acad family, lacks a TATA or CAAT box and is GC rich. This region does contain multiple, putative cis-ac...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Jan 1, 1982·Annals of the New York Academy of Sciences·G P Mannaerts, L J Debeer
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·T GulickD P Kelly
Dec 14, 1993·Biochimica Et Biophysica Acta·B E HainlineA W Strauss
Mar 31, 1993·Biochemical and Biophysical Research Communications·T AoyamaT Hashimoto
Dec 15, 1995·Cell·D J MangelsdorfR M Evans
Mar 15, 1996·Science·G Schatz, B Dobberstein
Apr 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·C L Kelly, P A Wood
Jun 15, 1996·The Journal of Clinical Investigation·M RodenG I Shulman
Jan 1, 1996·Molecular Reproduction and Development·D K Barnett, B D Bavister
Jan 1, 1996·Annual Review of Cell and Developmental Biology·T LembergerW Wahli

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Citations

Mar 28, 2002·ILAR Journal·A Michele Schuler, Philip A Wood
May 18, 1999·Current Opinion in Lipidology·P A Wood
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·D M KurtzP A Wood
May 26, 2009·Biochimica Et Biophysica Acta·Malika ChegarySander M Houten
Feb 6, 2004·Physiological Genomics·Matthias BauerJoerg D Katzenberger
May 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Riikka J LundRiitta Lahesmaa

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