Structural characterization of the N-terminal mineral modification domains from the molluscan crystal-modulating biomineralization proteins, AP7 and AP24

Biopolymers
Brandon A WustmanJohn Spencer Evans

Abstract

The AP7 and AP24 proteins represent a class of mineral-interaction polypeptides that are found in the aragonite-containing nacre layer of mollusk shell (H. rufescens). These proteins have been shown to preferentially interfere with calcium carbonate mineral growth in vitro. It is believed that both proteins play an important role in aragonite polymorph selection in the mollusk shell. Previously, we demonstrated the 1-30 amino acid (AA) N-terminal sequences of AP7 and AP24 represent mineral interaction/modification domains in both proteins, as evidenced by their ability to frustrate calcium carbonate crystal growth at step edge regions. In this present report, using free N-terminal, C(alpha)-amide "capped" synthetic polypeptides representing the 1-30 AA regions of AP7 (AP7-1 polypeptide) and AP24 (AP24-1 polypeptide) and NMR spectroscopy, we confirm that both N-terminal sequences possess putative Ca (II) interaction polyanionic sequence regions (2 x -DD- in AP7-1, -DDDED- in AP24-1) that are random coil-like in structure. However, with regard to the remaining sequences regions, each polypeptide features unique structural differences. AP7-1 possesses an extended beta-strand or polyproline type II-like structure within the A11-M10...Continue Reading

References

Nov 1, 1992·Journal of Biomolecular NMR·M PiottoV Sklenár
Nov 20, 1991·Journal of Molecular Biology·D S WishartF M Richards
May 5, 1988·Journal of Molecular Biology·H J DysonR A Lerner
Sep 1, 1995·Journal of Biomolecular NMR·D S WishartB D Sykes
Jan 8, 2000·The Journal of Biological Chemistry·V GerbaudJ M Verdier
Mar 4, 2003·Nature Materials·Siqun WangAnand Jagota
Sep 3, 2003·Nature Materials·Mehmet SarikayaFrançois Baneyx

❮ Previous
Next ❯

Citations

Apr 15, 2006·Marine Biotechnology·Cen Zhang, Rongqing Zhang
Dec 11, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·B GaumeS Auzoux-Bordenave
Apr 5, 2017·Ecology·Richard K ZimmerJoseph A Loo
Aug 7, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·X-L WangZ-Y Fu
Mar 10, 2017·Acta Biomaterialia·Ethan Michael GerhardJian Yang
Oct 22, 2008·Chemical Reviews·M Cusack, A Freer
Jun 19, 2008·Biomacromolecules·Sebastiano Collino, John Spencer Evans
Feb 7, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Rebecca A MetzlerP U P A Gilbert
Dec 1, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·John L KulpJohn Spencer Evans
Dec 15, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Il Won KimJohn Spencer Evans
Mar 27, 2007·Biomacromolecules·Sebastiano Collino, John Spencer Evans
Feb 21, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·John L KulpJohn Spencer Evans

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.