Structural characterization of the Pseudomonas aeruginosa 1244 pilin glycan

The Journal of Biological Chemistry
P CastricR W Carlson

Abstract

An antigenic similarity between lipopolysaccharide (LPS) and glycosylated pilin of Pseudomonas aeruginosa 1244 was noted. We purified a glycan-containing molecule from proteolytically digested pili and showed it to be composed of three sugars and serine. This glycan competed with pure pili and LPS for reaction with an LPS-specific monoclonal antibody, which also inhibited twitching motility by P. aeruginosa bearing glycosylated pili. One-dimensional NMR analysis of the glycan indicated the sugars to be 5N beta OHC(4)7NfmPse, Xyl, and FucNAc. The complete proton assignments of these sugars as well as the serine residue were determined by COSY and TOCSY. Electrospray ionization mass spectrometry (MS) determined the mass of this molecule to be 771.5. The ROESY NMR spectrum, tandem MS/MS analysis, and methylation analysis provided information on linkage and the sequence of oligosaccharide components. These data indicated that the molecule had the following structure: alpha-5N beta OHC(4)7NFmPse-(2-->4)beta-Xyl-(1-->3)-beta-FucNAc-(1-->3)-beta-Ser.

References

Oct 1, 1979·Canadian Journal of Microbiology·W ParanchychT H Watts
Aug 18, 1976·Journal of Chromatography·M GhebregzabherM Lato
Jul 1, 1977·Journal of Bacteriology·L S Frost, W Paranchych
Apr 1, 1991·Infection and Immunity·R RamphalS Lory
Jan 1, 1990·Annual Review of Biochemistry·C R Raetz
Jan 1, 1990·Critical Reviews in Microbiology·Y A Knirel
Mar 1, 1989·Molecular & General Genetics : MGG·P A CastricJ C Sadoff
Jan 1, 1989·Annual Review of Biochemistry·J Lechner, F Wieland
Jan 1, 1988·Advances in Microbial Physiology·W Paranchych, L S Frost
Feb 1, 1980·Canadian Journal of Microbiology·D E Bradley
Mar 1, 1983·Reviews of Infectious Diseases·G P BodeyL Jadeja
Jan 1, 1993·Annual Review of Microbiology·M S Strom, S Lory
Jan 1, 1993·The Journal of Infectious Diseases·A S CrossJ C Sadoff
Jan 8, 1999·Biochimica Et Biophysica Acta·P Burda, M Aebi
Jun 11, 1999·Molecular Microbiology·C M SzymanskiP Guerry
Dec 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·M E Davey, G A O'toole
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·F WielandW Schaefer

❮ Previous
Next ❯

Citations

Dec 13, 2003·Carbohydrate Research·Evgeny VinogradovWilliam W Kay
Mar 13, 2014·Journal of Electroanalytical Chemistry·Binod PandeyKeith J Stine
Dec 9, 2003·Trends in Microbiology·M Alexander SchmidtInga Benz
Feb 15, 2003·FEMS Microbiology Letters·P M Power, M P Jennings
Dec 21, 2002·Diagnostic Microbiology and Infectious Disease·Ana C GalesUNKNOWN SENTRY Participants Group (Latin America)
Sep 7, 2013·Nature Protocols·Madhu Emmadi, Suvarn S Kulkarni
Mar 2, 2005·Nature Reviews. Microbiology·Christine M Szymanski, Brendan W Wren
Oct 16, 2010·Nature Reviews. Microbiology·Harald Nothaft, Christine M Szymanski
Apr 22, 2004·Nature Reviews. Microbiology·Lisa CraigJohn A Tainer
Jul 19, 2002·Molecular Microbiology·Inga Benz, M Alexander Schmidt
Sep 14, 2011·The Journal of Biological Chemistry·Gerald PoschChristina Schäffer
Nov 16, 2005·The Journal of Biological Chemistry·Joseph HorzempaPeter Castric
Oct 22, 2008·The Journal of Biological Chemistry·Amirreza FaridmoayerMario F Feldman
Mar 16, 2002·Infection and Immunity·Christine M SzymanskiPatricia Guerry
May 16, 2002·Infection and Immunity·Jason E ComerPeter Castric
Nov 22, 2005·Infection and Immunity·James G SmedleyPeter Castric
Sep 14, 2010·Journal of Bacteriology·Mohammed QutyanPeter Castric
Jun 3, 2006·Journal of Bacteriology·Joseph HorzempaPeter Castric
Aug 2, 2011·Journal of Bacteriology·Rebecca M ThomasJoann L Prior
Sep 25, 2007·Journal of Bacteriology·Amirreza FaridmoayerMario F Feldman
May 24, 2011·Journal of Bacteriology·Andrew E ScottJoann L Prior
Jan 11, 2011·Journal of Bacteriology·Kai-Malte BartelsFrank Rosenau
Jun 3, 2006·Journal of Bacteriology·Amrisha VermaReuben Ramphal
Aug 19, 2003·Journal of Bacteriology·Vesna SimunovicLawrence J Shimkets
Apr 20, 2004·Journal of Bacteriology·Paul Messner
Sep 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·Leslie CuthbertsonChris Whitfield
Dec 4, 2012·Microbiology and Molecular Biology Reviews : MMBR·Carmen L GiltnerLori L Burrows
Sep 20, 2006·Journal of Molecular Microbiology and Biotechnology·Sandy Y M NgKen F Jarrell
Oct 11, 2011·PloS One·Patrick S HopfCarole Creuzenet
Feb 11, 2010·Future Microbiology·Christopher W ReidSusan M Twine
Oct 31, 2002·Molecular Microbiology·Antonio DiGiandomenicoPeter Castric

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.