Structural characterization of three novel rat OKL38 transcripts, their tissue distributions, and their regulation by human chorionic gonadotropin

Endocrinology
Choon Kiat OngH Huynh

Abstract

We previously identified a novel pregnancy-induced growth inhibitory gene, OKL38. To develop a rat model for further characterization of OKL38's role in the initiation and progression of breast and ovarian cancer, we now report the cloning and characterization of three novel rat OKL38 cDNAs that are derived through alternative splicing and differential promoter usage. These three transcripts differ in their 5' untranslated regions but share a common open reading frame that encoded for a 52-kDa protein. OKL38 is mapped to chromosome 19, spanning a region of approximately 15 kb, and contains eight exons. Differential expression of these three rat OKL38 transcripts was observed in liver, kidney, ovary, mammary gland, and uterus. In situ hybridization localized the rat OKL38 transcripts to the luminal epithelial cells of the rat mammary gland and to the granulosa cells in the rat ovary. In vivo studies showed that the RtOKL38-2.0 transcript and protein were regulated by human chorionic gonadotropin in the rat mammary gland and ovary. Importantly, overexpression of RtOKL38-enhanced green fluorescence protein fusion protein in Buffalo rat liver cells resulted in growth inhibition and cell death. Our present findings suggest that OKL3...Continue Reading

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Citations

May 24, 2008·The Journal of Biological Chemistry·Hongjie YaoYanming Wang
Aug 2, 2005·Biochemical and Biophysical Research Communications·Jason MatthewsJan-Ake Gustafsson
Dec 29, 2006·Journal of Lipid Research·Rongsong LiJudith A Berliner
Jan 31, 2020·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Gonca Yetkin YildirimIsmail Dağ
Jun 12, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Chia-Han TsaiChien-Chun Li

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