PMID: 8604311Feb 15, 1996Paper

Structural characterization of U*-1915 in domain IV from Escherichia coli 23S ribosomal RNA as 3-methylpseudouridine

Nucleic Acids Research
J A KowalakJames A McCloskey

Abstract

Mass spectrometry-based methods have been used to study post-transcriptional modification in the 1900-1974 nt segment of domain IV in 23S rRNA of Escherichia coli, a region which interacts with domain V in forming the three- dimensional structure of the peptidyl transferase center within the ribosome. A nucleoside constituent of M r 258 (U*)which occurs at position 1915, within the highly modified oligonucleotide sequence 1911-psiAACU*Apsi-1917, was characterized as 3-methylpseudouridine (m3psi). The assignment was confirmed by chemical synthesis of m3psi and comparison with the natural nucleoside by liquid chromatography-mass spectrometry. 3-Methylpseudouridine is previously unknown in nature and is the only known derivative of the common modified nucleoside pseudouridine thus far found in bacterial rRNA.

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Citations

Jan 1, 1996·Mass Spectrometry Reviews·E NordhoffP Roepstorff
Feb 5, 2013·Bioorganic & Medicinal Chemistry·Moninderpal KaurChristine S Chow
Mar 21, 1998·Current Opinion in Biotechnology·P F Crain, J A McCloskey
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