Structural colocalisation of type VI collagen and fibronectin in agarose cultured chondrocytes and isolated chondrons extracted from adult canine tibial cartilage

Journal of Anatomy
J ChangC A Poole

Abstract

Cell-matrix and matrix-matrix interactions are of critical importance in regulating the development, maintenance and repair of articular cartilage. In this study, we examined the structural colocalisation of type VI collagen and fibronectin in isolated chondrons and long-term agarose cultured chondrocytes extracted from normal adult canine articular cartilage. Using double labelling immunohistochemistry in conjunction with dual channel confocal microscopy and digital image processing we demonstrate that type VI collagen and fibronectin are distributed in a similar staining pattern and are colocalised at the surface of cultured chondrocytes and isolated chondrons. The results suggest that type VI collagen and fibronectin may play a role in both cell-matrix adhesion and matrix-matrix cohesion in the pericellular microenvironment surrounding articular cartilage chondrocytes.

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Citations

Aug 6, 2011·International Journal of Rheumatic Diseases·Carla M ThomasMohammed Sharif
Jul 31, 2008·PloS One·Gidon OfekKyriacos A Athanasiou
Dec 7, 2007·The Journal of Cell Biology·Leontine L Galante, Jean E Schwarzbauer
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May 26, 2007·Osteoarthritis and Cartilage·M A DimiccoA J Grodzinsky
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Jul 1, 1997·Osteoarthritis and Cartilage·G M LeeB Caterson
Jun 22, 2005·Progress in Histochemistry and Cytochemistry·C W JonesM H Zheng

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