Structural comparison of prostate-specific antigen and human glandular kallikrein using molecular modeling

Urology
D P Bridon, B Dowell

Abstract

Prostate-specific antigen (PSA), the most useful tumor marker for prostate cancer, is one of three members of the human kallikrein family of serine proteases. PSA and human glandular kallikrein (hK2, previously called hGK-1) share extensive homology and are both produced in the prostate under androgen control. Our goals were to use molecular modeling techniques to generate models of the tertiary structure of PSA and hK2 and to compare their molecular features and areas of homology using these models. Models of PSA and hK2 were generated by extrapolating from available crystallographic coordinates and amino acid sequences of homologous members of the serine protease family using standard comparative methods. Porcine kallikrein (57% homology) and rat tonin (53% homology) were used as templates for PSA. Porcine kallikrein (67% homology) was used as a template for hK2. The models were superimposed to define regions of nonhomology between PSA and hK2. Three-dimensional protein models of PSA and hK2 were generated. These models have potential uses in analyzing antigen-antibody interactions, modeling of inhibitor complexes of both PSA and hK2, and furthering our understanding of the molecular interactions involved in the clinical dete...Continue Reading

References

May 25, 1977·Journal of Molecular Biology·F C BernsteinM Tasumi
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K W WattR Loor
Apr 20, 1987·FEBS Letters·A Lundwall, H Lilja
Oct 8, 1987·The New England Journal of Medicine·T A StameyE Redwine
Apr 28, 1989·Biochemical and Biophysical Research Communications·P Henttu, P Vihko
Oct 1, 1987·DNA·L J SchedlichB J Morris
Nov 15, 1994·Biochemical and Biophysical Research Communications·M Vihinen
Nov 1, 1994·Protein Science : a Publication of the Protein Society·B O VilloutreixJ H Griffin
Jan 1, 1994·Critical Reviews in Biochemistry and Molecular Biology·M S JohnsonT L Blundell

❮ Previous
Next ❯

Citations

May 1, 1996·Protein Science : a Publication of the Protein Society·B O VilloutreixO Teleman
Jul 1, 1997·Protein Science : a Publication of the Protein Society·D E Timm
Jan 3, 1997·Clinica Chimica Acta; International Journal of Clinical Chemistry·C Becker, H Lilja
Jun 1, 1997·European Journal of Biochemistry·S D MikolajczykM S Saedi
Nov 28, 2001·Protein Expression and Purification·Y WangD S Wishart
Nov 23, 2000·Protein Expression and Purification·K K BowmanH Zhan
Aug 17, 2004·Critical Reviews in Clinical Laboratory Sciences·Judith A ClementsYing Dong
Oct 6, 1998·Critical Reviews in Clinical Laboratory Sciences·H G RittenhouseA W Partin
Jul 20, 2002·The Journal of Urology·A G Kennedy-SmithD N J Hart
Sep 30, 1998·Chemistry & Biology·G S CoombsD R Corey

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.