Structural conservation of the PIN domain active site across all domains of life

Protein Science : a Publication of the Protein Society
M SenissarDitlev E Brodersen

Abstract

The PIN (PilT N-terminus) domain is a compact RNA-binding protein domain present in all domains of life. This 120-residue domain consists of a central and parallel β sheet surrounded by α helices, which together organize 4-5 acidic residues in an active site that binds one or more divalent metal ions and in many cases has endoribonuclease activity. In bacteria and archaea, the PIN domain is primarily associated with toxin-antitoxin loci, consisting of a toxin (the PIN domain nuclease) and an antitoxin that inhibits the function of the toxin under normal growth conditions. During nutritional or antibiotic stress, the antitoxin is proteolytically degraded causing activation of the PIN domain toxin leading to a dramatic reprogramming of cellular metabolism to cope with the new situation. In eukaryotes, PIN domains are commonly found as parts of larger proteins and are involved in a range of processes involving RNA cleavage, including ribosomal RNA biogenesis and nonsense-mediated mRNA decay. In this review, we provide a comprehensive overview of the structural characteristics of the PIN domain and compare PIN domains from all domains of life in terms of structure, active site architecture, and activity.

References

May 15, 1973·Journal of Molecular Biology·S T Rao, M G Rossmann
Oct 24, 1997·Science·R L TatusovD J Lipman
Apr 27, 1999·Molecular Microbiology·D Wall, D Kaiser
Jan 4, 2001·Current Biology : CB·P M Clissold, C P Ponting
Apr 5, 2003·Current Biology : CB·Patrick ReichenbachJoachim Lingner
Apr 18, 2003·Current Biology : CB·Bryan E SnowLea Harrington
Jan 22, 2004·The Journal of Biological Chemistry·Vickery L ArcusEdward N Baker
Sep 25, 2004·RNA·Alessandro FaticaMensur Dlakić
Feb 19, 2005·Nucleic Acids Research·Deo Prakash Pandey, Kenn Gerdes
Feb 22, 2005·Molecular Cell·Noemi FukuharaElena Conti
May 3, 2005·Nature Reviews. Microbiology·Kenn GerdesAnders Løbner-Olesen
May 20, 2005·Current Opinion in Cell Biology·Elena Conti, Elisa Izaurralde
Mar 3, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Jeyaraman JeyakanthanTahir H Tahirov
Jun 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Franziska BleichertSusan J Baserga
Oct 13, 2006·The Journal of Biological Chemistry·Susanne RötherKatja Strässer
Nov 23, 2006·RNA Biology·Vivek Anantharaman, L Aravind
Jun 9, 2007·Proteins·Daijiro TakeshitaMasaru Tanokura
Oct 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Hong-Wei WangAilong Ke
Dec 9, 2008·Nature·Alice LebretonBertrand Séraphin
Dec 9, 2008·Nature Structural & Molecular Biology·Andrea B EberleTorben Heick Jensen
Dec 9, 2008·Nature Structural & Molecular Biology·Daneen SchaefferAmbro van Hoof
Jan 16, 2009·Biochemical Society Transactions·Charlotte R CooperRobert M Kelly
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Allison C Lamanna, Katrin Karbstein
Nov 19, 2009·Nucleic Acids Research·Robert D FinnAlex Bateman
Oct 12, 2010·Nucleic Acids Research·Yucheng ShaoZixin Deng
Apr 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kristoffer S Winther, Kenn Gerdes
May 25, 2011·Extremophiles : Life Under Extreme Conditions·Xiao-Yan YouRoger A Garrett

❮ Previous
Next ❯

Citations

Mar 12, 2019·Current Drug Targets·Weiwei KeXiangxuan Zhao
May 11, 2018·Scientific Reports·Mateusz WilamowskiJolanta Jura
Dec 18, 2019·Cells·Tatiana KamaletdinovaZhao-Qi Wang
May 5, 2020·Frontiers in Genetics·Dukas Jurėnas, Laurence Van Melderen
Oct 3, 2020·International Journal of Molecular Sciences·Richard MussonJolanta Jura
Sep 19, 2020·Science·Jingdong ChengRoland Beckmann
Dec 24, 2019·Journal of Molecular Biology·Haser H SutcuAlexander A Ishchenko

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.