Structural contributions to the intracellular targeting strategies of antimicrobial peptides.

Biochimica Et Biophysica Acta
Yun LanA James Mason

Abstract

The interactions of cationic amphipathic antimicrobial peptides (AMPs) with anionic biological membranes have been the focus of much research aimed at improving the activity of such compounds in the search for therapeutic leads. However, many of these peptides are thought to have other polyanions, such as DNA or RNA, as their ultimate target. Here a combination of fluorescence and circular dichroism (CD) spectroscopies has been used to assess the structural properties of amidated versions of buforin II, pleurocidin and magainin 2 that support their varying abilities to translocate through bacterial membranes and bind to double stranded DNA. Unlike magainin 2 amide, a prototypical membrane disruptive AMP, buforin II amide adopts a poorly helical structure in membranes closely mimicking the composition of Gram negative bacteria, such as Escherichia coli, and binds to a short duplex DNA sequence with high affinity, ultimately forming peptide-DNA condensates. The binding affinities of the peptides to duplex DNA are shown to be related to the structural changes that they induce. Furthermore, CD also reveals the conformation of the bound peptide buforin II amide. In contrast with a synthetic peptide, designed to adopt a perfect amphi...Continue Reading

References

Jan 1, 1980·Annual Review of Biophysics and Bioengineering·I TinocoM F Maestre
Feb 23, 1994·Biochimica Et Biophysica Acta·S J LudtkeH W Huang
Dec 1, 1993·Protein Science : a Publication of the Protein Society·B BechingerS J Opella
Oct 29, 1996·Biochemistry·S J LudtkeH W Huang
Nov 25, 1996·FEBS Letters·G S YiC Cheong
Feb 19, 2002·Antimicrobial Agents and Chemotherapy·Aleksander PatrzykatRobert E W Hancock
Aug 15, 2002·Biochimica Et Biophysica Acta·Nathalie SaintGérard Molle
Nov 24, 2004·International Journal of Antimicrobial Agents·K V R ReddyC Aranha
Dec 8, 2004·Biochemistry·Satoe KobayashiKatsumi Matsuzaki
Feb 11, 2005·Nature Reviews. Microbiology·Kim A Brogden
Jul 16, 2005·Current Eye Research·Y Jerold GordonAlison M McDermott
Mar 18, 2006·Biochimica Et Biophysica Acta·Huey W Huang
Jun 7, 2006·Molecular Membrane Biology·A James MasonBurkhard Bechinger
Aug 8, 2006·Current Opinion in Pharmacology·Alexandra K MarrRobert Ew Hancock
Sep 14, 2006·Journal of the American Chemical Society·Hari LeontiadouSiewert J Marrink
Dec 13, 2006·Nature Biotechnology·Robert E W Hancock, Hans-Georg Sahl
Nov 28, 2007·Expert Review of Anti-infective Therapy·John D F Hale, Robert E W Hancock
May 2, 2008·FEBS Letters·Erika T UyterhoevenDonald E Elmore
May 3, 2008·Proteins·Eleanor FlemingDonald E Elmore
Oct 2, 2008·Journal of the American Chemical Society·Richard M EpandRaquel F Epand
Dec 2, 2008·Biochimica Et Biophysica Acta·Ju Hyun ChoSun Chang Kim
Mar 26, 2009·Critical Care Medicine·Oscar CirioniAndrea Giacometti

❮ Previous
Next ❯

Citations

Jan 17, 2012·Progress in Lipid Research·Vitor TeixeiraMargarida Bastos
Jun 18, 2011·Trends in Biotechnology·Leonard T NguyenHans J Vogel
Feb 20, 2014·BioMed Research International·Irina KerkisTetsuo Yamane
Jan 10, 2012·Biochimica Et Biophysica Acta·Valentina IacobucciA James Mason
Dec 10, 2015·Proteomics·Pramod ShahChien-Sheng Chen
Mar 15, 2015·Biochimica Et Biophysica Acta·Thitiporn AnunthawanSompong Klaynongsruang
Apr 9, 2013·Biochimica Et Biophysica Acta·Evan F HaneyHans J Vogel
Jul 6, 2016·Frontiers in Microbiology·Sudheer GuptaVineet K Sharma
Sep 25, 2016·Microbiological Research·Filippo FratiniAntonio Felicioli
Oct 26, 2018·Medicinal Research Reviews·Jiajun WangAnshan Shan
May 24, 2020·International Journal of Molecular Sciences·Pethaiah GunasekaranJeong Kyu Bang
Jan 30, 2019·Frontiers in Immunology·Joseph F A VargaBarbara A Katzenback
Jan 6, 2021·Advanced Drug Delivery Reviews·Yunjiang JiangJianjun Cheng
Mar 14, 2021·Colloids and Surfaces. B, Biointerfaces·Yuhan YanChunguang Ren
Jun 3, 2021·Pharmaceuticals·Malak PirtskhalavaGrigol Managadze
Jun 25, 2021·Journal of Chemical Information and Modeling·Pietro G A AronicaChandra S Verma
Jun 15, 2011·ACS Applied Materials & Interfaces·Shuilin WuPaul K Chu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.