Structural Delineation of the Neck Linker of Kinesin-3 for Processive Movement

Journal of Molecular Biology
Jinqi RenWei Feng

Abstract

Processive kinesin motors contain a neck linker (NL) that mediates the chemo-mechanical coupling and controls the directionality and processivity. However, kinesin-3 NL remains poorly determined due to the lack of the structural information of the junction with the following neck coil (NC). Here, we determined the structure of the motor domain (MD)-NL-NCNT tandem of KIF13B that defines the junction between NL and NC and delineates kinesin-3 NL. Unexpectedly, the length of kinesin-3 NL is much shorter than the previously predicted one. In the MD-NL-NCNT structure, NL docks onto the MD with a conventional mode but the interaction between NL and the MD is relatively weak due to the shorter N-terminal cover strand of the MD. The optimal short NL and its weak interaction with the MD would generate the tight inter-head strain and facilitate the NL undocking, which may contribute to the fast and superprocessive motility of kinesin-3.

Citations

May 31, 2020·Protein Science : a Publication of the Protein Society·Byron Hunter, John S Allingham
Nov 23, 2018·Proceedings of the National Academy of Sciences of the United States of America·Jinqi RenWei Feng
May 26, 2021·Current Biology : CB·Elias T Spiliotis, Konstantinos Nakos

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