Structural determinants of calcium signaling by RGD peptides in rat osteoclasts: integrin-dependent and -independent actions

Experimental Cell Research
G ShankarM A Horton

Abstract

Extensive characterization of the vitronectin receptor (VNR), a member of the integrin group of cell adhesion molecules, which is abundantly expressed in osteoclasts, has revealed a role for this receptor in osteoclast adhesion as well as bone resorption. Earlier evidence from our laboratory suggests that VNR is also capable of transducing intracellular signals following receptor ligand interaction, although this function is poorly understood. Thus, addition of peptides containing the minimal tripeptide Arg-Gly-Asp (RGD) integrin recognition sequence elicits transient increases in intracellular free calcium ions, with maximal responses seen with a bone sialoprotein peptide, BSP-IIA. In the present study we have attempted to determine some of the structural requirements for calcium signaling in osteoclasts using derivatives of the peptide PRGDN/T sequence found in bone sialoprotein. While some peptides, such as the parent sequence PRGDN, can induce both signaling and retractile events, it was found that minor structural modifications yielded peptides such as PRADN which elicited a transient increase in intracellular free calcium ions without promoting a reduction in osteoclast spread area (retraction). Conversely, certain other ...Continue Reading

Citations

Jul 27, 1999·Proceedings of the Association of American Physicians·M ZaidiO A Adebanjo
Aug 12, 2006·Canadian Journal of Physiology and Pharmacology·Dawidson A GomesMichael H Nathanson
Feb 28, 2008·Journal of Periodontal Research·Y Ogata
Apr 12, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·B GanssJ Sodek
Aug 2, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Paloma ValverdeJake Chen
Jan 1, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M D Sjaastad, W J Nelson
Aug 30, 2008·Journal of Cellular Physiology·Qisheng TuJake Chen
Mar 12, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yasuhito TeruiKiyohiko Hatake
Dec 10, 1999·Experimental Cell Research·W RaddingH C Blair
Mar 17, 2004·Biochemical and Biophysical Research Communications·Qisheng TuJake Jinkun Chen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.