Structural determinants of the PDE6 GAF A domain for binding the inhibitory gamma-subunit and noncatalytic cGMP

Vision Research
Hakim MuradovNikolai O Artemyev

Abstract

Photoreceptor cGMP phosphodiesterases (PDE6 family) are modular enzymes with each catalytic subunit containing two N-terminal regulatory GAF domains, GAF A and GAF B. The GAF A domains contribute to dimerization of the PDE6 catalytic subunits and to binding of the inhibitory Pgamma subunits, and represent candidate sites for noncatalytic binding of cGMP. We performed a mutational analysis of selected residues from the GAF A domain of cone PDEalpha' to identify the cGMP-binding pocket and delineate the Pgamma-binding surface. Results of this analysis establish the noncatalytic cGMP-binding site within the PDE6 GAF A domain and suggest that occupation of the pocket by cGMP is required for high-affinity binding of Pgamma to the proximate contact surface.

References

Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T S LiM L Applebury
Feb 1, 1989·European Journal of Biochemistry·M Chabre, P Deterre
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·P G Gillespie, J A Beavo
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P DeterreM Chabre
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A YamazakiM W Bitensky
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·R H CoteV Y Arshavsky
Jan 20, 1998·Trends in Biochemical Sciences·L Aravind, C P Ponting
Sep 12, 1998·The Journal of Biological Chemistry·A E GranovskyN O Artemyev
Mar 29, 2000·Methods in Enzymology·M Natochin, N O Artemyev
Sep 29, 2000·Progress in Nucleic Acid Research and Molecular Biology·S H FrancisJ D Corbin
Oct 12, 2000·The Journal of Biological Chemistry·A E Granovsky, N O Artemyev
Feb 5, 2002·Annual Review of Physiology·Vadim Y ArshavskyEdward N Pugh
Sep 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Sergio E MartinezJoseph A Beavo
Jan 30, 2003·The EMBO Journal·Sergei D RybalkinJoseph A Beavo
Jun 26, 2003·Nucleic Acids Research·Torsten SchwedeManuel C Peitsch

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Citations

Nov 1, 2005·Vision Research·Hakim MuradovNikolai O Artemyev
Apr 30, 2011·Physiological Reviews·Sharron H FrancisJackie D Corbin
Jan 26, 2005·The Journal of Biological Chemistry·Lian-Wang GuoArnold E Ruoho
Jul 30, 2011·The Journal of Biological Chemistry·Saravanan KolandaiveluVisvanathan Ramamurthy
Jan 1, 2011·The AFCS-nature Molecule Pages·Karyn B Cahill, Rick H Cote
Aug 21, 2020·The Journal of Biological Chemistry·Annika N BoehmAnnette Aichem
Mar 27, 2018·European Journal of Medicinal Chemistry·Ting PengJianyou Shi
Jul 6, 2010·Journal of Molecular Biology·Anna GocKrzysztof Palczewski

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