Structural determinants required to target penicillin-binding protein 3 to the septum of Escherichia coli

Journal of Bacteriology
André PietteMartine Nguyen-Distèche

Abstract

In Escherichia coli, cell division is mediated by the concerted action of about 12 proteins that assemble at the division site to presumably form a complex called the divisome. Among these essential division proteins, the multimodular class B penicillin-binding protein 3 (PBP3), which is specifically involved in septal peptidoglycan synthesis, consists of a short intracellular M1-R23 peptide fused to a F24-L39 membrane anchor that is linked via a G40-S70 peptide to an R71-I236 noncatalytic module itself linked to a D237-V577 catalytic penicillin-binding module. On the basis of localization analyses of PBP3 mutants fused to green fluorescent protein by fluorescence microscopy, it appears that the first 56 amino acid residues of PBP3 containing the membrane anchor and the G40-E56 peptide contain the structural determinants required to target the protein to the cell division site and that none of the putative protein interaction sites present in the noncatalytic module are essential for the positioning of the protein to the division site. Based on the effects of increasing production of FtsQ or FtsW on the division of cells expressing PBP3 mutants, it is suggested that these proteins could interact. We postulate that FtsQ could pl...Continue Reading

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Citations

Sep 29, 2011·Antimicrobial Agents and Chemotherapy·Rodrigo CayôLuis Martínez-Martínez
Aug 23, 2006·Journal of Bacteriology·S LeimanisJ Coyette
Apr 29, 2008·Journal of Bacteriology·Audrey Le GouëllecAndré Zapun
Oct 31, 2006·Journal of Bacteriology·Nathan W GoehringJon Beckwith
Dec 4, 2004·Journal of Bacteriology·Soumya PastoretMartine Nguyen-Distèche
Dec 13, 2005·Microbiology and Molecular Biology Reviews : MMBR·Dirk-Jan Scheffers, Mariana G Pinho
Oct 31, 2007·The Journal of Cell Biology·Matthew T Cabeen, Christine Jacobs-Wagner
Apr 27, 2010·Journal of Molecular Biology·Allison FayJonathan Dworkin
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Jun 29, 2006·Molecular Microbiology·Ute BertscheWaldemar Vollmer
Mar 4, 2008·Molecular Microbiology·Fusinita van den EntJan Löwe
Feb 13, 2008·FEMS Microbiology Reviews·Eric SauvagePaulette Charlier
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Oct 30, 2020·Microbiology and Molecular Biology Reviews : MMBR·Alexander Cambré, Abram Aertsen

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